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Title:
FLUORESCENCE DETECTION DEVICE AND FLUORESCENCE DETECTION METHOD
Document Type and Number:
WIPO Patent Application WO/2014/136784
Kind Code:
A1
Abstract:
A fluorescence detection device (1) is provided with the following: an excitation light source (10); a phase-modulating spatial light modulator (20) that can phase-modulate excitation light (L1), yielding phase-modulated light (L2); focusing optics (30) that are provided after the spatial light modulator (20) and focus the phase-modulated light (L2) onto a sample (90); a sample stage (92) onto which said sample (90) is placed; a fluorescence detector (40) that detects fluorescence (L3) produced as a result of the phase-modulated light (L2) being focused onto the sample (90); a control unit (21) that displays a first CGH on the spatial light modulator (20); and a correction unit (22) that corrects said first CGH. Said correction unit (22) is provided with the following: a detector-specific sensitivity-information storage unit (222) that, in advance, acquires and stores sensitivity information specific to the fluorescence detector (40) per detection position; and a second-hologram generation unit (223) that generates a second CGH by correcting the first CGH on the basis of the intensity of the fluorescence (L3) and the aforementioned sensitivity information. The control unit (21) displays said second CGH on the spatial light modulator (20).

Inventors:
MATSUMOTO NAOYA (JP)
Application Number:
JP2014/055491
Publication Date:
September 12, 2014
Filing Date:
March 04, 2014
Export Citation:
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Assignee:
HAMAMATSU PHOTONICS KK (JP)
International Classes:
G01N21/64; G02B21/06; G03H1/08
Domestic Patent References:
WO2004017069A12004-02-26
Foreign References:
JP2011128572A2011-06-30
JP2009300589A2009-12-24
JPH10186283A1998-07-14
JP2005292662A2005-10-20
Other References:
NIKOLENKO ET AL.: "SLM microscopy: scanless two-photon imaging and photostimulation with spatial light modulators", FRONTIERS IN NEURAL CIRCUITS, vol. 2, no. 5, 19 December 2008 (2008-12-19), pages 1 - 14, XP055146495, DOI: doi:10.3389/neuro.04.005.2008
SHAO Y ET AL.: "Ultrafast, large-field multiphoton microscopy based on anacousto-optic deflector and a spatial light modulator", OPTICS LETTERS, vol. 37, no. 13, 1 July 2012 (2012-07-01), pages 2532 - 2534, XP001576832, DOI: doi:10.1364/OL.37.002532
Attorney, Agent or Firm:
HASEGAWA Yoshiki et al. (JP)
Yoshiki Hasegawa (JP)
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