Title:
FLUORESCENCE INTENSITY AND LIFETIME DISTRIBUTION ANALYSIS
Document Type and Number:
WIPO Patent Application WO2001059436
Kind Code:
A3
Abstract:
The present invention relates to a method for characterizing samples having fluorescent particles, comprising the steps of exciting particles in a measurement volume to emit fluorescence by a series of excitation pulses, monitoring the emitted fluorescence by detecting sequences of photon counts using a detection means, determining numbers of photon counts in counting time intervals of given width, determining in said counting time intervals detection delay times of the photon counts relative to the corresponding excitation pulses, determining a function of said detection delay times, determining a probability function of at least two arguments, P(n,t,...), wherein at least one argument is the number of photon counts and another argument is said function of detection delay times, and determining from said probability function P(n,t,...) a distribution of particles as a function of at least two arguments, wherein one argument is a specific brightness of the particles, or a measure thereof, and another argument is a fluorescence lifetime of the particles, or a measure thereof.
Inventors:
KASK PEET (EE)
Application Number:
PCT/EP2001/001470
Publication Date:
March 14, 2002
Filing Date:
February 10, 2001
Export Citation:
Assignee:
EVOTEC AG (DE)
KASK PEET (EE)
KASK PEET (EE)
International Classes:
G01J3/44; G01N21/64; G01N37/00; G01N15/02; (IPC1-7): G01N21/64; G01J3/44
Foreign References:
| EP0884583A1 | 1998-12-16 | |||
| EP0836090A1 | 1998-04-15 |
Other References:
KASK P ET AL: "Two-Dimensional Fluorescence Intensity Distribution Analysis: Theory and Applications", BIOPHYSICAL JOURNAL, NEW YORK, US, US, vol. 78, April 2000 (2000-04-01), pages 1703 - 1713, XP002168069, ISSN: 0006-3495
SHUMING NIE ET AL: "REAL-TIME DETECTION OF SINGLE MOLECULES IN SOLUTION BY CONFOCAL FLUORESCENCE MICROSCOPY", ANALYTICAL CHEMISTRY, AMERICAN CHEMICAL SOCIETY. COLUMBUS, US, vol. 67, no. 17, 1 September 1995 (1995-09-01), pages 2849 - 2857, XP000647270, ISSN: 0003-2700
SHUMING NIE ET AL: "REAL-TIME DETECTION OF SINGLE MOLECULES IN SOLUTION BY CONFOCAL FLUORESCENCE MICROSCOPY", ANALYTICAL CHEMISTRY, AMERICAN CHEMICAL SOCIETY. COLUMBUS, US, vol. 67, no. 17, 1 September 1995 (1995-09-01), pages 2849 - 2857, XP000647270, ISSN: 0003-2700
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