Title:
FLUORESCENT PROBE FOR BRANCHED CHAIN AMINO ACIDS AND USE THEREOF
Document Type and Number:
WIPO Patent Application WO/2019/174633
Kind Code:
A1
Abstract:
Provided is a fluorescent probe, containing a) a response polypeptide and b) an optical active polypeptide, wherein the optical active polypeptide is inserted into the response polypeptide. Also provided is a nucleic acid sequence coding the fluorescent probe of any embodiment of the present invention or a complementary sequence thereof. Also provided is an expression vector containing the nucleic acid sequence of the present invention, which is operably linked to an expression control sequence, or the complementary sequence thereof. Also provided is a cell containing the expression vector of the present invention. Also provided is a method for preparing the fluorescent probe of the present invention, the method comprising the steps of: providing the cell containing the vector for expressing the fluorescent probe of the present invention; culturing the cell under the cell expression conditions; and separating the fluorescent probe. Also provided is a use of the fluorescent probe of the present invention or the fluorescent probe prepared by the method of the present invention in detecting branched chain amino acids.
In one embodiment, the branched chain amino acids are selected from leucine, isoleucine and valine. The detection can be performed in vivo, in vitro, at subcellular level or at in-situ level. Further provided is a kit containing the fluorescent probe of the present invention or the fluorescent probe prepared by the method of the present invention.
Inventors:
YANG YI (CN)
ZHAO YUZHENG (CN)
LI XIE (CN)
ZOU YEJUN (CN)
ZHANG CHANGCHENG (CN)
ZHAO YUZHENG (CN)
LI XIE (CN)
ZOU YEJUN (CN)
ZHANG CHANGCHENG (CN)
Application Number:
PCT/CN2019/078274
Publication Date:
September 19, 2019
Filing Date:
March 15, 2019
Export Citation:
Assignee:
UNIV EAST CHINA SCIENCE & TECH (CN)
International Classes:
C07K19/00; G01N21/64
Foreign References:
| CN108395484A | 2018-08-14 | |||
| CN105694851A | 2016-06-22 | |||
| CN106232623A | 2016-12-14 |
Other References:
DATABASE Genbank 31 January 2014 (2014-01-31), "Leu/Ile/Val-binding Protein Precursor[Escherichia Coli CFT073", XP55635222, Database accession no. AAN82689.1
DATABASE Genbank 25 July 2016 (2016-07-25), "Leu/Ile/Val-binding Protein (LIV-BP)[Escherichia Coli M718", XP55635225, Database accession no. EGI19136
TATEDA N ET AL., ANALYTICAL SCIENCES: THE INTERNATIONAL JOURNAL OF THE JAPAN SOCIETY FOR ANALYTICAL CHEMISTRY, vol. 17, no. 6, 2001, pages 775 - 778
WADUD S ET AL.: "Analytical technologies in the biomedical and life sciences", JOURNAL OF CHROMATOGRAPHY B, vol. 767, no. 2, 2002, pages 369 - 374
LI X-T ET AL., CHEM RES CHIN UNIV, vol. 29, no. 3, 2013, pages 434 - 438
MENG J ET AL., THE ANALYST, vol. 135, no. 7, 2010, pages 1592 - 1599
HORTALA MA ET AL., J AM CHEM SOC, vol. 125, no. 1, 2003, pages 20 - 21
PU F ET AL., ANAL CHEM, vol. 82, no. 19, 2010, pages 8211 - 8216
DU J ET AL., CHEMICAL COMMUNICATIONS, vol. 49, no. 47, 2013, pages 5399 - 5401
ENGESER M ET AL., CHEMICAL COMMUNICATIONS, vol. 13, 1999, pages 1191 - 1192
HEIM, R. ET AL., PROC NATL ACAD SCI U S A., vol. 91, no. 26, 1994, pages 12501 - 12504
NAGAI, T. ET AL., PROC NATL ACAD SCI U.S.A., vol. 98, no. 6, 2001, pages 3197 - 3202
ALTSCHUL, NUCLEIC ACIDS RES., vol. 25, 1977, pages 3389
ALTSCHUL, J. MOL BIOL., vol. 215, 1990, pages 403
J. SAMBROOKD. W. RUSSELL: "Molecular Cloning: A Laboratory Guide", August 2002, COLD SPRING HARBOR LABORATORY PRESS
FRESENI ET AL., CULTURE OF ANIMAL CELLS: A MANUAL OF BASIC TECHNIQUE
J.S. BONIFASSINM. DASSAULT ET AL., SHORT PROTOCOLS IN CELL BIOLOGY
NAGAI, T. ET AL., PROC NATL ACAD SCI USA., vol. 98, no. 6, 2001, pages 3197 - 3202
See also references of EP 3778650A4
DATABASE Genbank 25 July 2016 (2016-07-25), "Leu/Ile/Val-binding Protein (LIV-BP)[Escherichia Coli M718", XP55635225, Database accession no. EGI19136
TATEDA N ET AL., ANALYTICAL SCIENCES: THE INTERNATIONAL JOURNAL OF THE JAPAN SOCIETY FOR ANALYTICAL CHEMISTRY, vol. 17, no. 6, 2001, pages 775 - 778
WADUD S ET AL.: "Analytical technologies in the biomedical and life sciences", JOURNAL OF CHROMATOGRAPHY B, vol. 767, no. 2, 2002, pages 369 - 374
LI X-T ET AL., CHEM RES CHIN UNIV, vol. 29, no. 3, 2013, pages 434 - 438
MENG J ET AL., THE ANALYST, vol. 135, no. 7, 2010, pages 1592 - 1599
HORTALA MA ET AL., J AM CHEM SOC, vol. 125, no. 1, 2003, pages 20 - 21
PU F ET AL., ANAL CHEM, vol. 82, no. 19, 2010, pages 8211 - 8216
DU J ET AL., CHEMICAL COMMUNICATIONS, vol. 49, no. 47, 2013, pages 5399 - 5401
ENGESER M ET AL., CHEMICAL COMMUNICATIONS, vol. 13, 1999, pages 1191 - 1192
HEIM, R. ET AL., PROC NATL ACAD SCI U S A., vol. 91, no. 26, 1994, pages 12501 - 12504
NAGAI, T. ET AL., PROC NATL ACAD SCI U.S.A., vol. 98, no. 6, 2001, pages 3197 - 3202
ALTSCHUL, NUCLEIC ACIDS RES., vol. 25, 1977, pages 3389
ALTSCHUL, J. MOL BIOL., vol. 215, 1990, pages 403
J. SAMBROOKD. W. RUSSELL: "Molecular Cloning: A Laboratory Guide", August 2002, COLD SPRING HARBOR LABORATORY PRESS
FRESENI ET AL., CULTURE OF ANIMAL CELLS: A MANUAL OF BASIC TECHNIQUE
J.S. BONIFASSINM. DASSAULT ET AL., SHORT PROTOCOLS IN CELL BIOLOGY
NAGAI, T. ET AL., PROC NATL ACAD SCI USA., vol. 98, no. 6, 2001, pages 3197 - 3202
See also references of EP 3778650A4
Attorney, Agent or Firm:
SHANGHAI PATENT & TRADEMARK LAW OFFICE, LLC (CN)
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