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Title:
FLUORESCENT PROBE FOR DETECTING SULFENATED PROTEIN, PREPARATION METHOD THEREFOR, AND APPLICATION THEREOF
Document Type and Number:
WIPO Patent Application WO/2018/228079
Kind Code:
A1
Abstract:
The present invention discloses a fluorescent probe for detecting a sulfenated protein, having the following structural general formula (I). The fluorescent probe has good stability and can be preserved for long-term use. The fluorescent probe is highly sensitive to its environment, having no strong fluorescence in a hydrophilic environment, and producing strong fluorescence only after reaction with a sulfenated protein such that a fluorophore is in a hydrophobic environment in the protein structure, and thus is able to develop in situ in cells the sulfhydryl sulfenated protein. Furthermore, the fluorescent probe has good water solubility and a relatively large absorption-emission wavelength difference, and can be paired with a tryptophan residue in a protein to produce a fluorescence resonance energy transfer (FRET) effect in an extracellular buffer solution detection system, effectively avoiding interference of excitation light and emitted light in protein samples. The fluorescent probe can specifically quantitatively detect a sulfenated protein in complex biological samples, and has a good detection ability of signal-to-noise ratios, is highly sensitive, and has excellent selectivity, thereby realizing specific detection of sulfhydryl sulfenation modification of intracellular proteins.

Inventors:
HU, Xiaolei (No. 1 Yixueyuan Road, YuZhong District, Chongqing 6, 400016, CN)
YANG, Xiaolan (No. 1 Yixueyuan Road, YuZhong District, Chongqing 6, 400016, CN)
YIN, Yibing (No. 1 Yixueyuan Road, YuZhong District, Chongqing 6, 400016, CN)
WU, Xiaomian (No. 1 Yixueyuan Road, YuZhong District, Chongqing 6, 400016, CN)
LIAO, Fei (No. 1 Yixueyuan Road, YuZhong District, Chongqing 6, 400016, CN)
LU, Jinpeng (No. 1 Yixueyuan Road, YuZhong District, Chongqing 6, 400016, CN)
LI, Juqiong (No. 1 Yixueyuan Road, YuZhong District, Chongqing 6, 400016, CN)
Application Number:
CN2018/085697
Publication Date:
December 20, 2018
Filing Date:
May 04, 2018
Export Citation:
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Assignee:
CHONGQING MEDICAL UNIVERSITY (No. 1 Yixueyuan Road, YuZhong District, Chongqing 6, 400016, CN)
International Classes:
C07C303/32; C07C309/47; C07C309/51; C07C315/04; C07C317/44; C09K11/06; G01N21/64
Foreign References:
CN107235866A2017-10-10
CN103102338A2013-05-15
CN1103533A1995-06-07
US4120892A1978-10-17
CN105693591A2016-06-22
Other References:
GHEE T. TAN ET AL.: "Potential Anti-AIDS Naphthalenesulfonic Acid Derivatives. Synthesis and Inhibition of HIV-1 Induced Cytopathogenesis and HIV-1 and HIV-2 Reverse Transcriptase Activities", J. MED. CHEM., vol. 35, no. 26, 31 December 1992 (1992-12-31), XP002005438
HUANG, CHUSEN ET AL.: "Chemical Approaches For Trapping Protein Thiols and Their Oxidative Modification", ACTA PHARMACEUTICA SINICA, vol. 47, no. 3, 31 December 2012 (2012-12-31)
HUANG, CHUSEN ET AL.: "Developing Fluorescent Probes to Trap Protein Vicinal Thiols and Sulfenylation in Living Cells", EAST CHINA UNIVERSITY OF SCIENCE AND TECHNOLOGY DOCTORATE PAPER, 31 December 2012 (2012-12-31)
Attorney, Agent or Firm:
HAIHONG JIACHENG INTELLECTUAL PROPERTY & PARTNERS (9th Floor, Tower B Zhongguancun Intellectual Property Mansion,No. 21, South Road Haidian, Haidian District, Beijing 0, 100080, CN)
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