Title:
FLUORESCENT PROBE, FLUORESCENCE DETECTION METHOD, AND METHOD FOR USING FLUORESCENT PROBE
Document Type and Number:
WIPO Patent Application WO/2017/187717
Kind Code:
A1
Abstract:
The present invention relates to a fluorescent probe which includes: a carrier molecule; and fluorescent dye a and fluorescent dye b which are bonded to the carrier molecule. Fluorescent dye a and fluorescent dye b have different excitation wavelengths. Fluorescence resonance energy transfer (FRET) does not occur between fluorescent dye a and fluorescent dye b. The present invention also relates to a fluorescence detection method which includes: a step in which target cells are labelled with the fluorescent probe; and a step in which the target cells labelled with the fluorescent probe are irradiated with excitation light, and fluorescence from the fluorescent probe is observed. Furthermore, the present invention relates to a method for using the fluorescent probe, said method including: a step in which cells are fluorescence labelled using the fluorescent probe; a step in which the fluorescence-labelled cells are screened using a flow cytometer or a fluorescence microscope; a step in which the screened fluorescence-labelled cells are transplanted into a living organism; and a step in which the fluorescence-labelled cells in the living organism are observed using an in vivo fluorescence imaging device.
Inventors:
HAYASHI KOICHIRO
SAKAMOTO WATARU
YOGO TOSHINOBU
MARUOKA HIROKI
SAKAMOTO WATARU
YOGO TOSHINOBU
MARUOKA HIROKI
Application Number:
PCT/JP2017/004981
Publication Date:
November 02, 2017
Filing Date:
February 10, 2017
Export Citation:
Assignee:
NAT UNIV CORP NAGOYA UNIV (JP)
KURASHIKI BOSEKI KK (JP)
KURASHIKI BOSEKI KK (JP)
International Classes:
G01N21/64; G01N15/14; G01N21/78; G01N33/48; G01N33/53; C12Q1/02
Domestic Patent References:
| WO2007097377A1 | 2007-08-30 | |||
| WO2015066552A1 | 2015-05-07 | |||
| WO2007064841A2 | 2007-06-07 |
Foreign References:
| JP2013535679A | 2013-09-12 | |||
| JP2012506439A | 2012-03-15 | |||
| JP2016014096A | 2016-01-28 |
Other References:
See also references of EP 3450963A4
Attorney, Agent or Firm:
IKEUCHI SATO & PARTNER PATENT ATTORNEYS (JP)
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