Title:
FLUORESCENT PROTEIN
Document Type and Number:
WIPO Patent Application WO/2017/155101
Kind Code:
A1
Abstract:
Provided is a greenish yellow fluorescent protein having high pH stability (reduced pH sensitivity). The fluorescent protein in one or a plurality of embodiments has an amino acid sequence in which at least the mutation below is introduced at the 2nd through 225th sequence of the amino acid sequence represented by SEQ ID NO: 1. Here, the mutation is selected from the group consisting of F149T or S or A, L158T or S or A, H160T or S or A, Y174T or S or A, Y192T or S or A, and combinations of 2 to 5 of the above mutations.
Inventors:
NAGAI TAKEHARU (JP)
SHINODA HAJIME (JP)
MATSUDA TOMOKI (JP)
MA YUANQING (AU)
SHINODA HAJIME (JP)
MATSUDA TOMOKI (JP)
MA YUANQING (AU)
Application Number:
PCT/JP2017/009759
Publication Date:
September 14, 2017
Filing Date:
March 10, 2017
Export Citation:
Assignee:
UNIV OSAKA (JP)
International Classes:
C07K19/00; C07K14/435; C12N1/15; C12N1/19; C12N1/21; C12N5/10; C12N15/09; G01N21/64; G01N21/78
Domestic Patent References:
| WO2011096501A1 | 2011-08-11 | |||
| WO2009020197A1 | 2009-02-12 |
Other References:
HAJIME SHINODA ET AL.: "1P0872: An acid insensitive green fluorescent protein from Olindias formosa'', ", DAI 88 KAI ANNUAL MEETING OF THE JAPANESE BIOCHEMICAL SOCIETY GODO TAIKAI) KOEN YOSHISHU, 2015, pages 1P0872, XP009515369
SHINODA, H. ET AL.: "Novel green fluorescent protein from Olindias formosa with exceptional pH stability", BIOPHYSICS, vol. 55, no. Suppl. 1-2, 2015, pages S298
TOMOKI MATSUDA ET AL.: "Tanryotai-gata Hikari Zokan Keiko Tanpakushitsu SuperNova", THE MOLECULAR BIOLOGY SOCIETY OF JAPAN DAI 37 KAI ANNUAL MEETING OF THE MOLECULAR BIOLOGY SOCIETY OF JAPAN KOEN YOSHISHU, vol. 37, 2014, XP009513280
See also references of EP 3428180A4
SHINODA, H. ET AL.: "Novel green fluorescent protein from Olindias formosa with exceptional pH stability", BIOPHYSICS, vol. 55, no. Suppl. 1-2, 2015, pages S298
TOMOKI MATSUDA ET AL.: "Tanryotai-gata Hikari Zokan Keiko Tanpakushitsu SuperNova", THE MOLECULAR BIOLOGY SOCIETY OF JAPAN DAI 37 KAI ANNUAL MEETING OF THE MOLECULAR BIOLOGY SOCIETY OF JAPAN KOEN YOSHISHU, vol. 37, 2014, XP009513280
See also references of EP 3428180A4
Attorney, Agent or Firm:
IKEUCHI SATO & PARTNER PATENT ATTORNEYS (JP)
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