Title:
FOKI NUCLEASE DOMAIN VARIANT
Document Type and Number:
WIPO Patent Application WO/2022/097663
Kind Code:
A1
Abstract:
It is found that four nuclease domain variants according to the present invention have nuclease activity superior to that of a wild type nuclease domain, and it is possible to improve genome editing efficiency in a combination of various nucleic acid-binding domains.
Inventors:
YAGI YUSUKE (JP)
OHTA MASARU (JP)
NAKAMURA TAKAHIRO (JP)
OHTA MASARU (JP)
NAKAMURA TAKAHIRO (JP)
Application Number:
PCT/JP2021/040503
Publication Date:
May 12, 2022
Filing Date:
November 04, 2021
Export Citation:
Assignee:
EDITFORCE INC (JP)
UNIV KYUSHU NAT UNIV CORP (JP)
UNIV KYUSHU NAT UNIV CORP (JP)
International Classes:
C12N1/15; C07K19/00; C12N1/19; C12N1/21; C12N5/10; C12N9/16; C12N15/55; C12N15/62
Domestic Patent References:
| WO2011091324A2 | 2011-07-28 | |||
| WO2018039448A1 | 2018-03-01 | |||
| WO2011097036A1 | 2011-08-11 | |||
| WO2003087341A2 | 2003-10-23 | |||
| WO2012104729A1 | 2012-08-09 | |||
| WO2011072246A2 | 2011-06-16 | |||
| WO2015019672A1 | 2015-02-12 | |||
| WO2014175284A1 | 2014-10-30 | |||
| WO2018221685A1 | 2018-12-06 | |||
| WO2014093712A1 | 2014-06-19 | |||
| WO2013176772A1 | 2013-11-28 | |||
| WO2013142578A1 | 2013-09-26 | |||
| WO2016205711A1 | 2016-12-22 | |||
| WO2018225858A1 | 2018-12-13 |
Foreign References:
| US20130217131A1 | 2013-08-22 |
Other References:
GUO, J. ET AL.: "Directed evolution of an enhanced and highly efficient fokI cleavage domain for zinc finger nucleases", J. MOL. BIOL., vol. 400, 2010, pages 96 - 107, XP055491626, DOI: 10.1016/j.jmb.2010.04.060
MILLER, J. C. ET AL.: "Enhancing gene editing specificity by attenuating DNA cleavage kinetics", NATURE BIOTECHNOLOGY, vol. 37, 2019, pages 945 - 952, XP036849997, DOI: 10.1038/s41587-019-0186-z
WAUGH, D. S. ET AL.: "A novel class of Fokl restriction endonuclease mutants that cleave hemi- methylated substrates", J. BIOL. CHEM., vol. 269, no. 16, 1994, pages 12298 - 12303, XP000971386
BITINAITE J ET AL., PROC NATL ACAD SCI U S A., vol. 95, 1998, pages 10570 - 10575
GUO J ET AL., J MOL BIOL, vol. 400, 2010, pages 96 - 107
DOYON Y ET AL., NAT METHODS, vol. 8, 2011, pages 74 - 79
KUNKEL, T.A., PROC NATL ACAD SCI USA, vol. 82, no. 2, 1985, pages 488 - 492
HASHIMOTO-GOTOH ET AL., GENE, vol. 152, 1995, pages 271 - 276
HO, S. N. ET AL., GENE, vol. 77, 1989, pages 51 - 59
NAKAMURA ET AL., PLANT CELL PHYSIOL, vol. 53, 2012, pages 1171 - 1179
BENJAMIN, P, NATURE, vol. 523, 2015, pages 481 - 485
HIRANO, S, MOLECULAR CELL, vol. 61, 2016, pages 886 - 894
STRECKER J ET AL., NATURE COMMUNICATIONS, vol. 10, 2019, pages 212
LIU JJ ET AL., NATURE, vol. 566, 2019, pages 218 - 223
J MOL BIOL., vol. 405, 2011, pages 630 - 641
SZCZEPEK M, NAT BIOTECHNOL., vol. 25, 2007, pages 786 - 793
MILLER JC ET AL., NAT BIOTECHNOL., vol. 37, 2019, pages 945 - 952
MILLER, J. C. ET AL.: "Enhancing gene editing specificity by attenuating DNA cleavage kinetics", NATURE BIOTECHNOLOGY, vol. 37, 2019, pages 945 - 952, XP036849997, DOI: 10.1038/s41587-019-0186-z
WAUGH, D. S. ET AL.: "A novel class of Fokl restriction endonuclease mutants that cleave hemi- methylated substrates", J. BIOL. CHEM., vol. 269, no. 16, 1994, pages 12298 - 12303, XP000971386
BITINAITE J ET AL., PROC NATL ACAD SCI U S A., vol. 95, 1998, pages 10570 - 10575
GUO J ET AL., J MOL BIOL, vol. 400, 2010, pages 96 - 107
DOYON Y ET AL., NAT METHODS, vol. 8, 2011, pages 74 - 79
KUNKEL, T.A., PROC NATL ACAD SCI USA, vol. 82, no. 2, 1985, pages 488 - 492
HASHIMOTO-GOTOH ET AL., GENE, vol. 152, 1995, pages 271 - 276
HO, S. N. ET AL., GENE, vol. 77, 1989, pages 51 - 59
NAKAMURA ET AL., PLANT CELL PHYSIOL, vol. 53, 2012, pages 1171 - 1179
BENJAMIN, P, NATURE, vol. 523, 2015, pages 481 - 485
HIRANO, S, MOLECULAR CELL, vol. 61, 2016, pages 886 - 894
STRECKER J ET AL., NATURE COMMUNICATIONS, vol. 10, 2019, pages 212
LIU JJ ET AL., NATURE, vol. 566, 2019, pages 218 - 223
J MOL BIOL., vol. 405, 2011, pages 630 - 641
SZCZEPEK M, NAT BIOTECHNOL., vol. 25, 2007, pages 786 - 793
MILLER JC ET AL., NAT BIOTECHNOL., vol. 37, 2019, pages 945 - 952
Attorney, Agent or Firm:
CENTCREST IP ATTORNEYS (JP)
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