Title:
FORMATE DEHYDROGENASE MUTANT, AND USE THEREOF
Document Type and Number:
WIPO Patent Application WO/2013/176157
Kind Code:
A1
Abstract:
A formate dehydrogenase mutant protein having a β-nicotinamide adenine dinucleotide phosphate-dependent formate dehydrogenase activity is constructed by introducing a site-specific mutation into a histidine residue located at position-224 in an amino acid sequence for a formate dehydrogenase mutant originated from Mycobacterium vaccae. The protein can exhibit a high formate dehydrogenase activity in the presence of an organic solvent. The mutant is useful for the production of an alcohol using a ketone as a starting material or the like.
Inventors:
UEDA MOMOKO (JP)
YAMAMOTO HIROAKI (JP)
HAYASHI MOTOKO (JP)
YAMAMOTO HIROAKI (JP)
HAYASHI MOTOKO (JP)
Application Number:
PCT/JP2013/064152
Publication Date:
November 28, 2013
Filing Date:
May 22, 2013
Export Citation:
Assignee:
DAICEL CORP (JP)
International Classes:
C12N15/09; C12N1/15; C12N1/19; C12N1/21; C12N5/10; C12N9/04; C12P7/02; C12P13/00; C12P19/36
Domestic Patent References:
| WO2012169341A1 | 2012-12-13 | |||
| WO2007139055A1 | 2007-12-06 |
Foreign References:
| JP2004041062A | 2004-02-12 | |||
| JP2002223776A | 2002-08-13 | |||
| JP2010537656A | 2010-12-09 |
Other References:
HOLSCH K ET AL.: "Enantioselective reduction of prochiral ketones by engineered bifunctional fusion proteins", BIOTECHNOL. APPL. BIOCHEM., vol. 56, no. 4, 2010, pages 131 - 140
WU W ET AL.: "Site-saturation mutagenesis of formate dehydrogenase from Candida bodinii creating effective NADP+-dependent FDH enzymes", JOURNAL OF MOLECULAR CATALYSIS B: ENZYMATIC, vol. 61, no. 3-4, 2009, pages 157 - 161
ANDREADELI A ET AL.: "Structure-guided alteration of coenzyme specificity of formate dehydrogenase by saturation mutagenesis to enable efficient utilization of NADP+", FEBS J., vol. 275, no. 15, 2008, pages 3859 - 3869
DATABASE DATABASE DDBJ/EMBL/GENBAN 6 October 2001 (2001-10-06), "Definition: Mycobacterium vaccae fdh gene for formate dehydrogenase,complete cds.", retrieved from http://www.ncbi.nlm.nih.gov/nuccore/ ab072394 accession no. B072394
GALKIN A ET AL.: "Cloning of formate dehydrogenase gene from a methanol-utilizing bacterium Mycobacterium vaccae N10", APPL. MICROBIOL. BIOTECHNOL., vol. 44, no. 3-4, 1995, pages 479 - 483
TISHKOV VI ET AL.: "Pilot scale production and isolation of recombinant NAD+- and NADP+ specific formate dehydrogenases", BIOTECHNOL. BIOENG., vol. 64, no. 2, 1999, pages 187 - 193
TISHKOV VI ET AL.: "Protein engineering of formate dehydrogenase", BIOMOL. ENG., vol. 23, no. 2-3, 2006, pages 89 - 110
BRAUN M ET AL.: "Novel whole-cell biocatalysts with recombinant hydroxysteroid dehydrogenases for the asymmetric reduction of dehydrocholic acid", APPL. MICROBIOL. BIOTECHNOL., vol. 95, no. 6, September 2012 (2012-09-01), pages 1457 - 1468
HOELSCH K ET AL.: "Engineering of formate dehydrogenase: synergistic effect of mutations affecting cofactor specificity and chemical stability", APPL. MICROBIOL. BIOTECHNOL., vol. 97, no. 6, March 2013 (2013-03-01), pages 2473 - 2481
WU W ET AL.: "Site-saturation mutagenesis of formate dehydrogenase from Candida bodinii creating effective NADP+-dependent FDH enzymes", JOURNAL OF MOLECULAR CATALYSIS B: ENZYMATIC, vol. 61, no. 3-4, 2009, pages 157 - 161
ANDREADELI A ET AL.: "Structure-guided alteration of coenzyme specificity of formate dehydrogenase by saturation mutagenesis to enable efficient utilization of NADP+", FEBS J., vol. 275, no. 15, 2008, pages 3859 - 3869
DATABASE DATABASE DDBJ/EMBL/GENBAN 6 October 2001 (2001-10-06), "Definition: Mycobacterium vaccae fdh gene for formate dehydrogenase,complete cds.", retrieved from http://www.ncbi.nlm.nih.gov/nuccore/ ab072394 accession no. B072394
GALKIN A ET AL.: "Cloning of formate dehydrogenase gene from a methanol-utilizing bacterium Mycobacterium vaccae N10", APPL. MICROBIOL. BIOTECHNOL., vol. 44, no. 3-4, 1995, pages 479 - 483
TISHKOV VI ET AL.: "Pilot scale production and isolation of recombinant NAD+- and NADP+ specific formate dehydrogenases", BIOTECHNOL. BIOENG., vol. 64, no. 2, 1999, pages 187 - 193
TISHKOV VI ET AL.: "Protein engineering of formate dehydrogenase", BIOMOL. ENG., vol. 23, no. 2-3, 2006, pages 89 - 110
BRAUN M ET AL.: "Novel whole-cell biocatalysts with recombinant hydroxysteroid dehydrogenases for the asymmetric reduction of dehydrocholic acid", APPL. MICROBIOL. BIOTECHNOL., vol. 95, no. 6, September 2012 (2012-09-01), pages 1457 - 1468
HOELSCH K ET AL.: "Engineering of formate dehydrogenase: synergistic effect of mutations affecting cofactor specificity and chemical stability", APPL. MICROBIOL. BIOTECHNOL., vol. 97, no. 6, March 2013 (2013-03-01), pages 2473 - 2481
Attorney, Agent or Firm:
HARUNA, Masao et al. (JP)
Masao Haruna (JP)
Masao Haruna (JP)
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