Title:
FORMATION AND USE OF NEURONAL NETWORK, AND NEURON SEEDING DEVICE
Document Type and Number:
WIPO Patent Application WO/2014/045618
Kind Code:
A1
Abstract:
Provided are a culture device for neuronal network formation that makes it possible to construct a neuronal network while restricting movement of neurons grown in the culture medium, and a means for the use thereof. A culture device for neuronal network formation in which is formed a cell fixation part surrounded by a plurality of projections on a flat substrate that can be filled with cell culture medium, and in which (1) there are established spaces having widths such that the neuron cells cannot pass through between the plurality of projections, (2) the inner diameter is such that the cell fixation part can house the cells of from one to a number of neurons, (3) the substrate surface, which is the bottom of the cell fixation part, is coated by an extracellular matrix-forming substance and/or provided with minute through-holes for suctioning culture medium by a suction device on the lower side of the substrate surface.
Inventors:
URISU TSUNEO (JP)
WANG ZHI-HONG (JP)
UNO HIDETAKA (JP)
SAITO MIHO (JP)
NAGAOKA YASUTAKA (JP)
WANG ZHI-HONG (JP)
UNO HIDETAKA (JP)
SAITO MIHO (JP)
NAGAOKA YASUTAKA (JP)
Application Number:
PCT/JP2013/057976
Publication Date:
March 27, 2014
Filing Date:
March 21, 2013
Export Citation:
Assignee:
JAPAN SCIENCE & TECH AGENCY (JP)
International Classes:
C12M3/00; C12M1/34; C12N5/0793
Domestic Patent References:
| WO2005001018A1 | 2005-01-06 | |||
| WO2005116242A1 | 2005-12-08 | |||
| WO2007116978A1 | 2007-10-18 |
Foreign References:
| JP2004166692A | 2004-06-17 | |||
| JP2009204407A | 2009-09-10 | |||
| JP2009156572A | 2009-07-16 | |||
| JP2011500119A | 2011-01-06 | |||
| JP4567936B2 | 2010-10-27 | |||
| JPH0775547A | 1995-03-20 | |||
| JP2010227012A | 2010-10-14 | |||
| JP2010227087A | 2010-10-14 | |||
| JP2011193758A | 2011-10-06 |
Other References:
ZECK, G. ET AL.: "Noninvasive neuroelectronic interfacing with synaptically connected snail neurons immobilized on a semiconductor chip", PNAS, vol. 98, no. 18, 2001, pages 10457 - 10462, XP002305989
G. ZECK ET AL., PNAS, vol. 98, 2001, pages 10457 - 10462
J. ERICKSON ET AL., J. NEUROSCI. METHODS, vol. 175, 2008, pages 1 - 16
F.W. PFRIEGER ET AL., SCIENCE, vol. 277, 1997, pages 1684 - 1687
K.S. WILCOX ET AL., SYNAPSE, vol. 18, 1994, pages 128 - 151
See also references of EP 2899262A4
G. ZECK ET AL., PNAS, vol. 98, 2001, pages 10457 - 10462
J. ERICKSON ET AL., J. NEUROSCI. METHODS, vol. 175, 2008, pages 1 - 16
F.W. PFRIEGER ET AL., SCIENCE, vol. 277, 1997, pages 1684 - 1687
K.S. WILCOX ET AL., SYNAPSE, vol. 18, 1994, pages 128 - 151
See also references of EP 2899262A4
Attorney, Agent or Firm:
KITAGAWA Osamu et al. (JP)
Kitagawa 治 (JP)
Kitagawa 治 (JP)
Download PDF: