Title:
FORMULATION AND USE OF PRESERVATION SOLUTION FOR CLINICAL CELL PREPARATIONS
Document Type and Number:
WIPO Patent Application WO/2020/227843
Kind Code:
A1
Abstract:
A preservation solution for clinical cell preparations, comprising the following concentration of ingredients: 100-500 of potassium dihydrogen phosphate; 300-900 of disodium hydrogen phosphate; 3000-9000 of sodium chloride; 3000-15000 of glucose; 1000-6000 of extracellular cryoprotectant; and 1000-6000 of intracellular cryoprotectant, the remainder being water, and the unit of concentration of the ingredients being mg/L. A preservation solution for clinical cell preparations, containing dual protective components, protecting the cells from both inside and outside against the damage of ice crystals during cryopreservation, greatly increasing the survival rate of the cells, and adapting to a wide variety of cell line preservations; in addition, the preservation solution for clinical cell preparations is a completely serum-free, protein-free, and DMSO-free cryopreservation solution, avoiding the heterogeneous exogenous ingredients introduced in clinical applications, and being safer.
Inventors:
LI HAIFENG (CN)
Application Number:
PCT/CN2019/086345
Publication Date:
November 19, 2020
Filing Date:
May 10, 2019
Export Citation:
Assignee:
YOCON BIOLOGY TECH COMPANY (CN)
International Classes:
A01N1/02
Foreign References:
| CN1857312A | 2006-11-08 | |||
| CN105961374A | 2016-09-28 | |||
| CN107467014A | 2017-12-15 | |||
| CN105230610A | 2016-01-13 | |||
| CN109479870A | 2019-03-19 |
Other References:
CAI CHAOCAO , WANG CHENG , CHEN SHIJIU: "Clinical Application Progress in Cryopreservation of Allografts", MEDICAL RECAPITULATE, vol. 25, no. 4, 28 February 2019 (2019-02-28), pages 769 - 773, XP055753513, ISSN: 1006-2084, DOI: 10.3969/j.issn.1006-2084.2019.04.027
GULSUN ERDAG , ALI ERGOLU , JEFFREY R MORGEAN , MEHMET TONER: "Cryopreservation of fetal skin is improved by extracellular trehalose.", CRYOBIOLOGY, vol. 44, no. 3, 30 June 2002 (2002-06-30), pages 218 - 228, XP002245788, ISSN: 0011-2240, DOI: 10.1016/S0011-2240(02)00023-8
GULSUN ERDAG , ALI ERGOLU , JEFFREY R MORGEAN , MEHMET TONER: "Cryopreservation of fetal skin is improved by extracellular trehalose.", CRYOBIOLOGY, vol. 44, no. 3, 30 June 2002 (2002-06-30), pages 218 - 228, XP002245788, ISSN: 0011-2240, DOI: 10.1016/S0011-2240(02)00023-8
Attorney, Agent or Firm:
LIU, SHEN & ASSOCIATES (CN)
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