Title:
FRAME STRUCTURE-BASED NANOPARTICLE USED FOR MASS CYTOMETRY AND PREPARATION METHOD THEREFOR
Document Type and Number:
WIPO Patent Application WO/2023/005614
Kind Code:
A1
Abstract:
A frame structure-based nanoparticle used for mass cytometry and a preparation method therefor. The nanoparticle comprises metal ions, an antibody, and a coordination network framework formed by connecting organic ligands to each other by means of a linker, wherein the metal ions are bonded to the organic ligands; the linker is an organic linker having the functionality of n or a metal cluster having a coordination number of n, and n is greater than or equal to 2; and the antibody is connected to the organic ligands or the linker by means of a grafting agent. The nanoparticle can load a plurality of metal isotopes, the loading capacity is high, and a high-sensitivity metal detection signal having a plurality of available metal isotope channels can be provided in flow-type mass spectrometry detection, thereby achieving single-cell protein analysis and detection of simultaneous measurement of more cell parameters, and providing an important analysis tool for accurate diagnosis of diseases at the gene level and research and development detection of biomedicine.
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Inventors:
CHEN YUAN (CN)
ZHAO JUNJIE (CN)
LUO YINGWU (CN)
ZENG XUN (CN)
ZHAO JUNJIE (CN)
LUO YINGWU (CN)
ZENG XUN (CN)
Application Number:
PCT/CN2022/103548
Publication Date:
February 02, 2023
Filing Date:
July 04, 2022
Export Citation:
Assignee:
UNIV ZHEJIANG (CN)
International Classes:
G01N15/14; B82Y5/00; B82Y30/00; G01N27/62; G01N33/531; G01N33/543
Foreign References:
| CN113588523A | 2021-11-02 | |||
| CN112834411A | 2021-05-25 | |||
| CN112778474A | 2021-05-11 | |||
| CN112683985A | 2021-04-20 | |||
| CN107796748A | 2018-03-13 | |||
| CN113075114A | 2021-07-06 | |||
| US20110135571A1 | 2011-06-09 | |||
| US20080095857A1 | 2008-04-24 |
Other References:
YANG YU, CHUANQIANG SUN, ZISHAN GONG, RU YANG, YAN WANG: "Application of Mass Cytometry in Single-Cell Detection", CHINESE JOURNAL OF CELL BIOLOGY, vol. 42, no. 2, 29 February 2020 (2020-02-29), pages 358 - 368, XP093029265, DOI: 10.11844/cjcb.2020.02.0022
LI, YU ET AL.: "Multicomponent zirconium-based metal-orangic frameworks for impedimetric aptasensing of living cancer cells",, vol. 306, 20 December 2019 (2019-12-20), XP085995225, DOI: 10.1016/j.snb.2019.127608
CHO, H. ET AL.: "A metal-chelating polymer for chelating zirconium and its use in mass cytometry",, vol. 120, 3 August 2019 (2019-08-03), XP085894756, DOI: 10.1016/j.eurpolymj.2019.08.002
LI, YU ET AL.: "Multicomponent zirconium-based metal-orangic frameworks for impedimetric aptasensing of living cancer cells",
CHO, H. ET AL.: "A metal-chelating polymer for chelating zirconium and its use in mass cytometry",
Attorney, Agent or Firm:
HANGZHOU QIUSHI PATENT OFFICE CO., LTD. (CN)
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