FREEZER AND FREEZING METHOD

Title:

FREEZER AND FREEZING METHOD

Document Type and Number:

WIPO Patent Application WO/2016/158479

Kind Code:

A1

Abstract:

Provided are: a freezer for quickly vitrifying a subject to be frozen such as cells by using a spray flow containing ultralow-temperature solid microparticles in freezing so as to achieve a high cell survival rate at thawing; and a freezing method using same. The freezer for freezing a subject to be frozen such as cells, said freezer comprising: a cryopreservation container 60 that houses the subject to be frozen such as cells; and a spray part that continuously sprays a high-speed spray flow containing ultralow-temperature solid microparticles to the cryopreservation container 60 so as to freeze the subject to be frozen that is housed in the cryopreservation container. This freezer is provided with a support part 71 that supports the cryopreservation container 60 and a spray position-changing part 72 (spray position-changing means) that changes a spray position of the high-seed spray flow to the cryopreservation container supported by the support part 71.

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Inventors:

ISHIMOTO JUN (JP)

Application Number:

PCT/JP2016/058634

Publication Date:

October 06, 2016

Filing Date:

March 18, 2016

Export Citation:

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Assignee:

UNIV TOHOKU (JP)

International Classes:

C12M1/00; A23L3/375; A23L17/30; C12N1/04; C12N5/04; C12N5/0735; C12N5/078; C12N5/10

Domestic Patent References:

WO1997028402A1	1997-08-07
WO1999066271A1	1999-12-23
WO2007119892A1	2007-10-25
WO2011047380A2	2011-04-21
WO2010046949A1	2010-04-29
WO2014199705A1	2014-12-18

Foreign References:

JP2012217342A	2012-11-12
JP2008002715A	2008-01-10

Other References:

SANSINENA, M. ET AL.: "Comparison of heat transfer in liquid and slush nitrogen by numerical simulation of cooling rates for French straws used for sperm cryopreservation", THERIOGENOLOGY, vol. 77, no. 8, 1 May 2012 (2012-05-01), pages 1717 - 1721, XP055320330, ISSN: 0093-691X

Attorney, Agent or Firm:

EICHI Patent & Trademark Corp. (JP)
Patent business corporation Wisdom international patent firm (JP)

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