Title:
FRUCTOSE-1,6-DIPHOSPHATE OPTICAL PROBE, AND PREPARATION METHOD THEREFOR AND USE THEREOF
Document Type and Number:
WIPO Patent Application WO/2024/094002
Kind Code:
A1
Abstract:
The present invention relates to a fructose diphosphate optical probe. Specifically, provided in the present invention is a fructose diphosphate optical probe, which comprises a fructose-1,6-diphosphate sensitive polypeptide and an optically active polypeptide, wherein the optically active polypeptide is located within the sequence of a fructose-1,6-diphosphate sensitive polypeptide, the fructose-1,6-diphosphate sensitive polypeptide is a fructose-1,6-diphosphate binding protein or a functional variant thereof, and the optically active polypeptide is a fluorescent protein or a functional variant thereof. The fluorescent probe of the present invention has a large dynamic change in terms of fluorescence and a good specificity, and can be used for high-throughput and quantitative detection of fructose diphosphate inside and outside a cell.
Inventors:
YANG YI (CN)
ZHAO YUZHENG (CN)
YAO JING (CN)
ZOU YEJUN (CN)
ZHANG LIJUAN (CN)
CHEN YAN (CN)
LI XIE (CN)
ZHAO YUZHENG (CN)
YAO JING (CN)
ZOU YEJUN (CN)
ZHANG LIJUAN (CN)
CHEN YAN (CN)
LI XIE (CN)
Application Number:
PCT/CN2023/128488
Publication Date:
May 10, 2024
Filing Date:
October 31, 2023
Export Citation:
Assignee:
EAST CHINA UNIV OF SCIENCE AND TECHNOLOGY (CN)
International Classes:
C07K14/00; C07K19/00; C12N1/21; C12N15/62; C12N15/70; G01N21/00; G01N33/53; G01N33/533; G01N33/58; C12R1/19
Attorney, Agent or Firm:
SHANGHAI PATENT & TRADEMARK LAW OFFICE, LLC (Xuhui District, Shanghai 3, CN)
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