Title:
FUCOIDAN FOR THROMBOLYSIS, DERIVED FROM SEAWEED
Document Type and Number:
WIPO Patent Application WO/2012/102555
Kind Code:
A2
Abstract:
To study different types of thrombolytic agents, both thrombolysis and stimulation of fucoidan were studied in a mouse model of ferric chloride-induced arterial thrombosis and were compared with those of a heparin and tissue plasminogen activator (t-PA). Thrombosis was induced by applying a filter paper saturated with 5% FeCl3 to the left carotid artery. 20 minutes after complete occlusion, several test preparations including a fucoidan source from Undaria pinnatifida sporophylls were intravenously injected to each mouse group by various dosages of 10-200 mg/kg-1. To study the stimulation effect of fucoidan with respect to t-PA, fucoidan added in a predetermined amount of t-PA was injected by a dose of 1-10 mg/kg. Then, the time to reperfusion was measured. As a result, an occluded aorta was dose-dependently reperfused, and the reperfusion was observed at 37.5±12.4 minutes after the administration of 100 mg/kg-1 of unfractionated fucoidan. The time to reperfusion was retarded to 55.0±8.0 minutes when a large amount of fucoidan such as 1 g/kg-1 was administered to a mouse provided with low molecular weight fucoidan (LMWF).
Reperfusion occurred at 16.67 minutes after administration in a mouse of a control group provided with 20 mg/kg-1 of t-PA. On the contrary, reperfusion was not observed in an occluded mouse provided with heparin (p<0.01). The occluded artery was recovered without reperfusion at 17.2 minutes after injection if the least amount of fucoidan added in a predetermined amount of t-PA was provided. All the data show that fucoidan can serve as both a thrombolytic agent and a stimulant with respect to thrombolysis activation of t-PA in a thrombosis model, differently from heparin.
Inventors:
KIM JONG-KI (KR)
Application Number:
PCT/KR2012/000611
Publication Date:
August 02, 2012
Filing Date:
January 26, 2012
Export Citation:
Assignee:
KIM JONG-KI (KR)
NEWTREE CO LTD (KR)
NEWTREE CO LTD (KR)
International Classes:
A61K31/737; A61K36/37; A61P7/02; A61P9/00
Domestic Patent References:
| WO2007127298A2 | 2007-11-08 |
Foreign References:
| JP2010132633A | 2010-06-17 | |||
| KR20100004529A | 2010-01-13 | |||
| KR100729638B1 | 2007-06-19 |
Attorney, Agent or Firm:
LEE, Duck-Rog (Yeilpat Bldg.669-17, Yeoksam-dong, Gangnam-gu, Seoul 135-915, KR)
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Claims: