Title:
FULL BACTERIA SACCHARIFICATION METHOD FOR LIGNOCELLULOSE
Document Type and Number:
WIPO Patent Application WO/2020/034821
Kind Code:
A1
Abstract:
Provided is a full bacteria saccharification method for lignocellulose, comprising the following steps: (1) optimizing of a seed solution: performing passage domestication on a cellulosome-producing strain in a culture medium which employs glucose as a unique carbon source under anaerobic conditions, then inoculating the same into a glucose culture medium which has a pretreated lignocellulose raw material added thereto, and performing induced domestication; (2) pre-impregnating of the raw material: uniformly mixing the pretreated lignocellulose raw material and a culture medium solution; and (3) full bacteria saccharification: inoculating the optimized seed solution of full bacteria saccharification from step (1) into a reaction system after pre-impregnating, and performing a saccharification reaction so as to obtain liquid sugar containing the glucose. The present method has the advantages of the cycle in the seed culture phase being greatly shortened, the cost of the seed solution being reduced, the full bacteria saccharification cycle being shortened, and thus a foundation being laid for an industrialization process.
Inventors:
CUI QIU (CN)
LIU YAJUN (CN)
QI KUAN (CN)
LIU SHIYUE (CN)
LI RENMIN (CN)
FENG YINGANG (CN)
LIU YAJUN (CN)
QI KUAN (CN)
LIU SHIYUE (CN)
LI RENMIN (CN)
FENG YINGANG (CN)
Application Number:
PCT/CN2019/097813
Publication Date:
February 20, 2020
Filing Date:
July 26, 2019
Export Citation:
Assignee:
QINGDAO INST BIOENERGY & BIOPROCESS TECH CAS (CN)
International Classes:
C12P19/14; C12P19/02; C12R1/01; C12R1/145
Domestic Patent References:
| WO2014078716A1 | 2014-05-22 | |||
| WO2009143481A1 | 2009-11-26 |
Foreign References:
| CN109055459A | 2018-12-21 | |||
| CN101824395A | 2010-09-08 | |||
| CN101851650A | 2010-10-06 |
Other References:
YOAV, S. ET AL.: "How does Cellulosome Composition Influence Deconstruction of Lignocellulosic Substrates in Clostridium (Ruminiclostridium) Thermocellum DSM 1313?", BIOTECHNOLOGY FOR BIOFUELS, vol. 10, no. 222, 31 December 2017 (2017-12-31), pages 1 - 16, XP055686456
HAROUN, B.M. ET AL.: "Significance of Acclimatization for Biohydrogen Production from Synthetic Lignocellulose Hydrolysate in Continuous-flow Systems", INTERNATIONAL JOURNAL OF HYDROGEN ENERGY, vol. 41, no. 32, 23 June 2016 (2016-06-23), pages 14003 - 14014, XP055364183, DOI: 10.1016/j.ijhydene.2016.05.289
HAROUN, B.M. ET AL.: "Significance of Acclimatization for Biohydrogen Production from Synthetic Lignocellulose Hydrolysate in Continuous-flow Systems", INTERNATIONAL JOURNAL OF HYDROGEN ENERGY, vol. 41, no. 32, 23 June 2016 (2016-06-23), pages 14003 - 14014, XP055364183, DOI: 10.1016/j.ijhydene.2016.05.289
Attorney, Agent or Firm:
QINGDAO HECHUANG INTELLECTUAL PROPERTY AGENCY FIRM (GENERAL PARTNERSHIP ) (CN)
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