Title:
FULLY-INTEGRATED PATHOGEN NUCLEIC ACID TEST MICROFLUIDIC CHIP
Document Type and Number:
WIPO Patent Application WO/2022/174471
Kind Code:
A1
Abstract:
Disclosed is a fully-integrated pathogen nucleic acid test microfluidic chip, comprising a fluid pipeline layer, an elastic adhesive tape layer and a reagent storage layer, wherein the reagent storage layer is configured for respectively storing chambers for collection liquid, cleaning liquid, a reaction reagent and waste liquid, an in-situ extraction amplification chamber having capture filter paper is provided in the fluid pipeline layer, the elastic adhesive tape layer is sandwiched between the fluid pipeline layer and the reagent storage layer, and the collection liquid, the cleaning liquid and the reaction reagent in the reagent storage layer pass through the elastic adhesive tape layer to flow to the fluid pipeline layer under the action of an external force, and flow to the in-situ extraction amplification chamber for reaction through a flow channel of the fluid pipeline layer. The fully-integrated pathogen nucleic acid test microfluidic chip of the present invention can integrate pathogen sample treatment, nucleic acid extraction and in-situ amplification into the same microfluidic chip device, such that complex operations and dependence on a professional laboratory during an actual pathogen nucleic acid test are eliminated.
Inventors:
LIU PENG (CN)
LI BAO (CN)
LI SHANGLIN (CN)
LIN BAOBAO (CN)
LI BAO (CN)
LI SHANGLIN (CN)
LIN BAOBAO (CN)
Application Number:
PCT/CN2021/078003
Publication Date:
August 25, 2022
Filing Date:
February 26, 2021
Export Citation:
Assignee:
HANGZHOU ZIJING BIOLOGY CO LTD (CN)
International Classes:
C12M1/34; B01L3/00; C12M1/00; C12M1/04
Foreign References:
| CN107541452A | 2018-01-05 | |||
| CN107502544A | 2017-12-22 | |||
| CN109852530A | 2019-06-07 | |||
| CN102253022A | 2011-11-23 | |||
| CN110452802A | 2019-11-15 | |||
| CN106085842A | 2016-11-09 |
Attorney, Agent or Firm:
JEEKAI&PARTNERS (CN)
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