Title:
FUNCTION-MODIFIED PHENYLALANINE DEHYDROGENASE, AND METHOD FOR ANALYSIS OF AMINO ACID IN BIOLOGICAL SAMPLE USING THE ENZYME
Document Type and Number:
WIPO Patent Application WO/2008/029921
Kind Code:
A1
Abstract:
A modified enzyme having such modification of at least three amino acid residues
that the modified enzyme can have improved substrate specificity of phenylalanine
dehydrogenase (EC 1.4.1.20) for methionine; DNA encoding the modified enzyme;
a method for the preparation of a modified enzyme which has such modification
of at least three amino acid residues that the modified enzyme can have improved
substrate specificity of phenylalanine dehydrogenase (EC 1.4.1.20) for methionine,
comprising the steps of culturing a transformant transformed with a vector carrying
the DNA, and collecting the modified enzyme from the culture; and a method for
the analysis of L-methionine contained in a sample by using the modified enzyme
or protein. A phenylalanine dehydrogenase having a substrate specificity for
L-methionine can be produced by an evolutionary molecular engineering approach
to provide a function-modified amino acid dehydrogenase suitable for enzymatic
fluorometry of methionine. It becomes possible to provide a method for the analysis
of L-methionine contained in a sample (a blood sample) by using the function-modified
amino acid dehydrogenase.
Inventors:
ASANO, Yasuhisa (4-803, 6 Sengoku-machi 2-chome, Toyama-sh, Toyama 66, 9300066, JP)
浅野 泰久 (〒66 富山県富山市千石町２丁目６番地４－８０３号 Toyama, 9300066, JP)
浅野 泰久 (〒66 富山県富山市千石町２丁目６番地４－８０３号 Toyama, 9300066, JP)
Application Number:
JP2007/067527
Publication Date:
March 13, 2008
Filing Date:
September 07, 2007
Export Citation:
Assignee:
TOYAMA PREFECTURE (1-7 Shinsogawa, Toyama-shi Toyama, 01, 9308501, JP)
富山県 (〒01 富山県富山市新総曲輪１番７号 Toyama, 9308501, JP)
TOYAMA NEW INDUSTRY ORGANIZATION (529 Takata, Toyama-shi Toyama, 66, 9300866, JP)
財団法人富山県新世紀産業機構 (〒66 富山県富山市高田５２９ Toyama, 9300866, JP)
富山県 (〒01 富山県富山市新総曲輪１番７号 Toyama, 9308501, JP)
TOYAMA NEW INDUSTRY ORGANIZATION (529 Takata, Toyama-shi Toyama, 66, 9300866, JP)
財団法人富山県新世紀産業機構 (〒66 富山県富山市高田５２９ Toyama, 9300866, JP)
International Classes:
C12N15/09; C07K19/00; C12N1/15; C12N1/19; C12N1/21; C12N5/10; C12N9/04; C12Q1/32; C12N15/09; C07K19/00; C12N1/15; C12N1/19; C12N1/21; C12N5/10; C12N9/04; C12Q1/32
Attorney, Agent or Firm:
SIKs & Co. (8th Floor, Kyobashi-Nisshoku Bldg.8-7, Kyobashi 1-chome,Chuo-k, Tokyo 31, 1040031, JP)