Title:
FUNCTIONAL LAYERS OF BIOMOLECULES AND LIVING CELLS, AND A NOVEL SYSTEM TO PRODUCE SUCH
Document Type and Number:
WIPO Patent Application WO/2010/040648
Kind Code:
A3
Abstract:
The present invention concerns a new process for depositing a thick compact layer of biomolecules for instance such a layer with thickness in the μm scale and, for depositing a thick compact layer of cells in the μm scale. The deposited layer is made by application of an unbalanced (asymmetrical) alternating voltage polarization between two electrodes to a dissolved biomolecule or cell from low conductivity solutions. The process allows the rapid manufacturing of sensors and the coating of devices with functional cells and biomolecules. Examples are provided on the preparation of functional sensors such as a glucose sensor is described in details, a hydrogen peroxide sensor and a glutamate sensor. Examples are also provided on the deposition of eukaryoric cells such as saccharomyces cerevisiae. The examples demonstrate a process that can be applied to coat devices with biomolecules and biological cells.
Inventors:
FRANSAER JAN (BE)
AMMAM MALIKA (BE)
AMMAM MALIKA (BE)
Application Number:
PCT/EP2009/062471
Publication Date:
April 28, 2011
Filing Date:
September 25, 2009
Export Citation:
Assignee:
UNIV LEUVEN KATH (BE)
FRANSAER JAN (BE)
AMMAM MALIKA (BE)
FRANSAER JAN (BE)
AMMAM MALIKA (BE)
International Classes:
C25D13/04; C12Q1/00; C25D13/12; C25D13/18; C25D13/22
Domestic Patent References:
| WO2002033150A2 | 2002-04-25 |
Foreign References:
| US5855753A | 1999-01-05 | |||
| US5872443A | 1999-02-16 | |||
| EP1132500A2 | 2001-09-12 | |||
| US6814845B2 | 2004-11-09 | |||
| US3200058A | 1965-08-10 | |||
| US5166063A | 1992-11-24 | |||
| US20060065531A1 | 2006-03-30 | |||
| DD215338A1 | 1984-11-07 |
Attorney, Agent or Firm:
BIRD, Ariane et al. (Klein Dalenstraat 42A, Winksele, BE)
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