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Title:
FUNGAL MICROASSAY METHOD USING SINGLE-CELL SEQUENCING AND KIT
Document Type and Number:
WIPO Patent Application WO/2018/006458
Kind Code:
A1
Abstract:
A fungal microassay method using single-cell sequencing and a fungal assay kit prepared using the method. The method comprises the steps: acquiring a small amount of fungal cells; performing cell disruption of the fungal cells to extract a fungal protoplasm; extracting and amplifying gDNA from a small amount of the fungal protoplasm; establishing a gDNA library; performing genome sequencing; performing bioinformatics analysis and comparison; and determining the species of a detected fungus. The method realizes a highly efficient fungal microassay, and can be directly used for separating, detecting, identifying, and performing in-depth genetic information study of a microscale volume of a fungus or a mixed sample. A fungal assay method and kit are applicable for various fields including industrial production, environment monitoring, air testing, soil testing, water quality testing, food testing, pharmaceutical testing, cosmetic testing, health supplement testing, medical testing, and the like.

Inventors:
YANG, Ying (The Academy Of Military Medical Sciences, No. 27 Taiping Road Haidian District, Beijing 9, 100089, CN)
WANG, Shengqi (The Academy Of Military Medical Sciences, No. 27 Taiping Road Haidian District, Beijing 9, 100089, CN)
ZHOU, Zhe (The Academy Of Military Medical Sciences, No. 27 Taiping Road Haidian District, Beijing 9, 100089, CN)
WANG, Peng (The Academy Of Military Medical Sciences, No. 27 Taiping Road Haidian District, Beijing 9, 100089, CN)
LI, Zhen (The Academy Of Military Medical Sciences, No. 27 Taiping Road Haidian District, Beijing 9, 100089, CN)
LI, Zongwei (The Academy Of Military Medical Sciences, No. 27 Taiping Road Haidian District, Beijing 9, 100089, CN)
Application Number:
CN2016/093734
Publication Date:
January 11, 2018
Filing Date:
August 05, 2016
Export Citation:
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Assignee:
INSTITUTE OF RADIATION MEDICINE, ACADEMY OF MILITARY MEDICAL SCIENCES (YANG, YingThe Academy Of Military Medical Sciences, No. 27 Taiping Road, Haidian District, Beijing 9, 100089, CN)
International Classes:
C12Q1/68; C12Q1/04; C12R1/645
Attorney, Agent or Firm:
BEIJING CROWN & RIGHTS LAW FIRM (Jack Zhu, Rm. 1407-1408 Tower 1, Office Building, Henderson Center, No. 18, Jianguomennei Street,Dongcheng District, Beijing 5, 100005, CN)
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