FUSION PROTEIN BETWEEN ANTIGEN-BINDING MOLECULE AND STREPTAVIDIN VARIANT

WIPO Patent Application WO/2023/008515

A1

The present invention addresses the problem of providing a fusion protein that is for use in treatment or diagnosis of cancer and that is formed between a molecule recognizing a cancer cell or the like and a streptavidin variant. According to the present invention, provided is a fusion protein in which an antigen-binding molecule having a molecular weight of 20,000 or less is bound to, via a linker sequence, the N-terminal side and/or the C-terminal side of a streptavidin variant, said streptavidin variant containing an amino acid sequence derived from the amino acid sequence of core streptavidin described in SEQ ID NO: 17 (wherein the amino acid sequence of Pro-Ser-Ala-Ala-Ser at the C-terminal may be partially or totally deleted) by the following mutations (1) to (6) and having reduced immunogenicity compared to wild-type streptavidin. (1) A mutation in which tyrosine at the 10th amino acid residue is replaced by serine or threonine. (2) A mutation in which tyrosine at the 71st amino acid residue is replaced by alanine or serine. (3) A mutation in which arginine at the 72nd amino acid residue is replaced by lysine. (4) A mutation in which glutamic acid at the 89th amino acid residue is replaced by aspartic acid. (5) A mutation in which arginine at the 91st amino acid residue is replaced by lysine. (6) A mutation in which glutamic acid at the 104th amino acid residue is replaced by glutamine or asparagine.

TANAKA TOSHIYA (JP)
YAMASHITA TAKEFUMI (JP)
KODAMA TATSUHIKO (JP)
KANAI MOTOMU (JP)
YAMATSUGU KENZO (JP)
TATSUMI TOSHIFUMI (JP)
TAKAHASHI KAZUKI (JP)
SUGIYAMA AKIRA (JP)
TSUKAGOSHI MASANOBU (JP)
CHANSLER MICHAEL (JP)
NAKAI MASANORI (JP)

PCT/JP2022/029073

February 02, 2023

July 28, 2022

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UNIV TOKYO (JP)
SAVID THERAPEUTICS INC (JP)

C12N15/62; A61K31/409; A61K38/16; A61K47/66; A61K49/00; A61P35/00; C07K14/195; C07K16/30; C07K19/00; C12P21/02

WO2020138427A1	2020-07-02
WO2020032165A1	2020-02-13
WO2010095455A1	2010-08-26

JP6127045B2	2017-05-10
JP2017524659A	2017-08-31

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SIKS & CO. (JP)

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