Title:
FUSION PROTEIN COMPRISING MODIFIED ANTI-VEGFR2 (KDR) ANTIBODY AND USE THEREOF
Document Type and Number:
WIPO Patent Application WO/2023/136648
Kind Code:
A1
Abstract:
The present invention relates to a fusion protein comprising a modified anti-VEGFR2 (KDR) antibody having improved properties or an antigen-binding fragment thereof, and a use thereof. Specifically, the present invention pertains to a fusion protein, and a composition for diagnosis or treatment of an angiogenesis-associated disease, a composition for prevention or treatment of a tumor or cancer, and a composition for administration in combination with a therapeutic agent other than the fusion protein, each composition comprising the fusion protein, wherein the fusion protein has an anti-VEGFR2(KDR) antibody conjugated with a delta-like ligand 4 (DLL4)-binding protein domain at the terminus thereof, the anti-VEGFR2(KDR) antibody being derived from a human antibody binding specifically to VEGFR2/KDR by substituting VH1 in the heavy chain framework region (FR) with VH3 and VL1 in the light chain framework region (FR) with VK1 and inducing a mutation therein, thereby increasing the affinity of the antibody for the antigen.
Inventors:
NAM JU RYOUNG (KR)
LEE YOUNGAE (KR)
LEE WEON SUP (KR)
YOO JIN-SAN (KR)
LEE YOUNGAE (KR)
LEE WEON SUP (KR)
YOO JIN-SAN (KR)
Application Number:
PCT/KR2023/000631
Publication Date:
July 20, 2023
Filing Date:
January 13, 2023
Export Citation:
Assignee:
PHARMABCINE INC (KR)
International Classes:
C07K16/28; A61P35/00; C12N15/62; C12N15/63; G01N33/68
Foreign References:
| KR20130118892A | 2013-10-30 | |||
| US9102720B2 | 2015-08-11 |
Other References:
HULTGREN NAN W., FANG JENNIFER S., ZIEGLER MARY E., RAMIREZ RICARDO N., PHAN DUC T. T., HATCH MICHAELA M. S., WELCH-REARDON KATRIN: "Slug regulates the Dll4-Notch-VEGFR2 axis to control endothelial cell activation and angiogenesis", NATURE COMMUNICATIONS, vol. 11, no. 1, XP093078288, DOI: 10.1038/s41467-020-18633-z
KUO KUAN-LIN; LIN WEI-CHOU; HO I-LIN; CHANG HONG-CHIANG; LEE PING-YI; CHUNG YUAN-TING; HSIEH JU-TON; PU YEONG-SHIAU; SHI CHUNG-SHE: "2-methoxyestradiol induces mitotic arrest, apoptosis, and synergistic cytotoxicity with arsenic trioxide in human urothelial carcinoma cells.", PLOS ONE, PUBLIC LIBRARY OF SCIENCE, US, vol. 8, no. 8, 1 January 2013 (2013-01-01), US , pages e68703, XP009516057, ISSN: 1932-6203, DOI: 10.1371/journal.pone.0068703
YOKOTA YASUHIRO, HIROYUKI NAKAJIMA, YUKI WAKAYAMA, AKIRA MUTO, KOICHI KAWAKAMI, SHIGETOMO FUKUHARA, NAOKI MOCHIZUKI : "Endothelial Ca2+ oscillations reflect VEGFR signaling-regulated angiogenic capacity in vivo ", ELIFE, vol. 4, 20 November 2015 (2015-11-20), pages 1 - 26, XP093078300, DOI: 10.7554/eLife.08817
KUO KUAN-LIN; LIN WEI-CHOU; HO I-LIN; CHANG HONG-CHIANG; LEE PING-YI; CHUNG YUAN-TING; HSIEH JU-TON; PU YEONG-SHIAU; SHI CHUNG-SHE: "2-methoxyestradiol induces mitotic arrest, apoptosis, and synergistic cytotoxicity with arsenic trioxide in human urothelial carcinoma cells.", PLOS ONE, PUBLIC LIBRARY OF SCIENCE, US, vol. 8, no. 8, 1 January 2013 (2013-01-01), US , pages e68703, XP009516057, ISSN: 1932-6203, DOI: 10.1371/journal.pone.0068703
YOKOTA YASUHIRO, HIROYUKI NAKAJIMA, YUKI WAKAYAMA, AKIRA MUTO, KOICHI KAWAKAMI, SHIGETOMO FUKUHARA, NAOKI MOCHIZUKI : "Endothelial Ca2+ oscillations reflect VEGFR signaling-regulated angiogenic capacity in vivo ", ELIFE, vol. 4, 20 November 2015 (2015-11-20), pages 1 - 26, XP093078300, DOI: 10.7554/eLife.08817
Attorney, Agent or Firm:
LEE, Cheo Young et al. (KR)
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