Title:
FUSION PROTEIN AND USE METHOD THEREOF
Document Type and Number:
WIPO Patent Application WO/2023/011638
Kind Code:
A1
Abstract:
The present invention relates to a fusion protein and a use method thereof. The fusion protein comprises a first protein and a second protein; the first protein is Cas, such as Cas9, Cas12a, Cas12b, Cas12e and TALEN or ZFN; the second protein is an exonuclease or endonuclease, such as TREX2, TREX1, APE1, Artemis, CtIP, Exo1, Mre11, RAD1, RAD9, Tp53, WRN, exonuclease V, T5 exonuclease, or T7 exonuclease. The first protein and the second protein are directly linked or linked by a linker. The fusion protein of the present invention can ensure the genome stability during gene editing, and reduce the generation of chromosome structure variation during single gene or multi-gene editing.
Inventors:
HU JIAZHI (CN)
YIN JIANXING (CN)
YIN JIANXING (CN)
Application Number:
PCT/CN2022/110597
Publication Date:
February 09, 2023
Filing Date:
August 05, 2022
Export Citation:
Assignee:
UNIV BEIJING (CN)
International Classes:
C07K19/00; A61K35/17; A61P43/00; C12N5/10; C12N9/22; C12N15/62; C12N15/85; C12N15/867; C12N15/87
Domestic Patent References:
| WO2020146290A1 | 2020-07-16 | |||
| WO2016191684A1 | 2016-12-01 |
Foreign References:
| CN113481184A | 2021-10-08 | |||
| CN108136047A | 2018-06-08 | |||
| CN109415687A | 2019-03-01 | |||
| CN109311984A | 2019-02-05 | |||
| CN111448313A | 2020-07-24 | |||
| CN108472314A | 2018-08-31 | |||
| CA2999500A1 | 2017-03-30 | |||
| US20160304855A1 | 2016-10-20 |
Other References:
YIN JIANHANG, LIU MENGZHU, LIU YANG, WU JINCHUN, GAN TINGTING, ZHANG WEIWEI, LI YINGHUI, ZHOU YAXUAN, HU JIAZHI: "Optimizing genome editing strategy by primer-extension-mediated sequencing", CELL DISCOVERY, vol. 5, no. 1, 1 December 2019 (2019-12-01), XP055773402, DOI: 10.1038/s41421-019-0088-8
FELICITY ALLEN, LUCA CREPALDI, CLARA ALSINET, ALEXANDER J. STRONG, VITALII KLESHCHEVNIKOV, PIETRO DE ANGELI, PETRA PáLENí: "Predicting the mutations generated by repair of Cas9-induced double-strand breaks", NATURE BIOTECHNOLOGY, NATURE PUBLISHING GROUP US, NEW YORK, vol. 37, no. 1, New York, pages 64 - 72, XP055580701, ISSN: 1087-0156, DOI: 10.1038/nbt.4317
KANG XI-LIN, CHU DAN-DAN, SHAN BIN: "Progress in Research and Application of New Gene Editing TechnologyCRISPR-Cas System ", GUOWAI-YIYAO-KANGSHENGSU-FENCE : SHUANGYUEKAN = WORLD NOTES ON ANTIBIOTICS, ZHONGGUO-KANSHENGSU ZAZHISHE, CHENGDU, CHINA, vol. 41, no. 1, 15 January 2020 (2020-01-15), Chengdu, China , pages 35 - 41, XP009543282, ISSN: 1001-8751
FELICITY ALLEN, LUCA CREPALDI, CLARA ALSINET, ALEXANDER J. STRONG, VITALII KLESHCHEVNIKOV, PIETRO DE ANGELI, PETRA PáLENí: "Predicting the mutations generated by repair of Cas9-induced double-strand breaks", NATURE BIOTECHNOLOGY, NATURE PUBLISHING GROUP US, NEW YORK, vol. 37, no. 1, New York, pages 64 - 72, XP055580701, ISSN: 1087-0156, DOI: 10.1038/nbt.4317
KANG XI-LIN, CHU DAN-DAN, SHAN BIN: "Progress in Research and Application of New Gene Editing TechnologyCRISPR-Cas System ", GUOWAI-YIYAO-KANGSHENGSU-FENCE : SHUANGYUEKAN = WORLD NOTES ON ANTIBIOTICS, ZHONGGUO-KANSHENGSU ZAZHISHE, CHENGDU, CHINA, vol. 41, no. 1, 15 January 2020 (2020-01-15), Chengdu, China , pages 35 - 41, XP009543282, ISSN: 1001-8751
Attorney, Agent or Firm:
RZBC PATENT AGENCY (GENERAL PARTNERSHIP) (CN)
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