Title:
FUSION PROTEIN
Document Type and Number:
WIPO Patent Application WO/2012/118092
Kind Code:
A1
Abstract:
The purpose of the present invention is to provide a fusion protein which is useful for the treatment or prevention of RNA virus infections. Provided are: a fusion protein produced by fusing a polypeptide having an affinity for viral RNA of an RNA virus to an endoribonuclease capable of cleaving single-stranded RNA in a sequence-specific manner; a nucleic acid which encodes the fusion protein; a vector which carries the nucleic acid; a method of treating or preventing retrovirus infections, comprising a step of introducing the vector into a cell; and a method of inhibiting the budding of a retrovirus using a cell having the nucleic acid introduced therein and the fusion protein. This fusion protein can inhibit the budding (production) of retrovirus particles from a retrovirus-infected cell effectively.
Inventors:
INOUE KOICHI (JP)
CHONO HIDETO (JP)
YAMAKAWA SATOKO (JP)
KAWANO YASUHIRO (JP)
MINENO JUNICHI (JP)
CHONO HIDETO (JP)
YAMAKAWA SATOKO (JP)
KAWANO YASUHIRO (JP)
MINENO JUNICHI (JP)
Application Number:
PCT/JP2012/054967
Publication Date:
September 07, 2012
Filing Date:
February 28, 2012
Export Citation:
Assignee:
TAKARA BIO INC (JP)
INOUE KOICHI (JP)
CHONO HIDETO (JP)
YAMAKAWA SATOKO (JP)
KAWANO YASUHIRO (JP)
MINENO JUNICHI (JP)
INOUE KOICHI (JP)
CHONO HIDETO (JP)
YAMAKAWA SATOKO (JP)
KAWANO YASUHIRO (JP)
MINENO JUNICHI (JP)
International Classes:
C12N15/00; A61K48/00; A61P31/14; A61P31/18; C07K19/00; C12N1/15; C12N1/19; C12N1/21; C12N5/10; C12N7/04; C12N15/09
Domestic Patent References:
| WO2007020873A1 | 2007-02-22 | |||
| WO2008133137A1 | 2008-11-06 | |||
| WO2009051078A1 | 2009-04-23 |
Other References:
OKAMOTO, M. ET AL.: "RNA Bunkai Koso MazF ga HIV LTR Ryoiki no Shihaika de Hatsugen suru Retrovirus Vector Donyu Saibo ni Okeru HIV-1 Teikosei no Kakutoku", THE JOURNAL OF AIDS RESEARCH, vol. 10, no. 4, 2008, pages P-090
SCHUMANN, G. ET AL.: "Therapeutic effect of a gag-nuclease fusion protein against retroviral infection in vivo", JOURNAL OF VIROLOGY, vol. 75, no. 15, August 2001 (2001-08-01), pages 7030 - 7041
NATSOULIS, G. ET AL.: "Targeting of a nuclease to murine leukemia virus capsids inhibits viral multiplication", PROC. NATL. ACAD. SCI. USA, vol. 92, January 1995 (1995-01-01), pages 364 - 368
SCHUMANN, G. ET AL.: "Antiretroviral effect of a gag-RNase HI fusion gene", GENE THERAPY, vol. 4, 1997, pages 593 - 599
KOYANAGI, Y.: "Outline of the HIV Replication and its Cellular Factors: the Track of an Invader in Cell", VIRUS, vol. 55, no. 2, 2005, pages 251 - 257
MURAKAMI, T. ET AL.: "Regulation of GAG trafficking and functions", THE JOURNAL OF AIDS RESEARCH, vol. 9, no. 2, 2007, pages 102 - 107
SAKURAGI, J.: "Structure and Function of HIV Virion Hoso Shutsuga kara Kyuchaku Shinnyu made", VIRUS, vol. 52, no. 1, 2002, pages 95 - 102
SCHUMANN, G. ET AL.: "Therapeutic effect of a gag-nuclease fusion protein against retroviral infection in vivo", JOURNAL OF VIROLOGY, vol. 75, no. 15, August 2001 (2001-08-01), pages 7030 - 7041
NATSOULIS, G. ET AL.: "Targeting of a nuclease to murine leukemia virus capsids inhibits viral multiplication", PROC. NATL. ACAD. SCI. USA, vol. 92, January 1995 (1995-01-01), pages 364 - 368
SCHUMANN, G. ET AL.: "Antiretroviral effect of a gag-RNase HI fusion gene", GENE THERAPY, vol. 4, 1997, pages 593 - 599
KOYANAGI, Y.: "Outline of the HIV Replication and its Cellular Factors: the Track of an Invader in Cell", VIRUS, vol. 55, no. 2, 2005, pages 251 - 257
MURAKAMI, T. ET AL.: "Regulation of GAG trafficking and functions", THE JOURNAL OF AIDS RESEARCH, vol. 9, no. 2, 2007, pages 102 - 107
SAKURAGI, J.: "Structure and Function of HIV Virion Hoso Shutsuga kara Kyuchaku Shinnyu made", VIRUS, vol. 52, no. 1, 2002, pages 95 - 102
Attorney, Agent or Firm:
SAMEJIMA, Mutsumi et al. (JP)
Mutsumi Sameshima (JP)
Mutsumi Sameshima (JP)
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