Title:
GENE ENCODING OF ENZYME FOR REDUCING POSITION 24 OF STEROID SKELETON AND PLANT HAVING REDUCED EXPRESSION OF SAME GENE
Document Type and Number:
WIPO Patent Application WO/2013/141383
Kind Code:
A1
Abstract:
The purpose of the invention is to provide a solanaceous plant (Solanaceae) that does not produce cholesterols, including glycoalkaloids. The invention is a protein having enzyme activity for reducing position 24 of a steroid skeleton in a solanaceous plant, a novel plant in which a gene encoding this protein is suppressed, and a method for creating and testing this plant.
Inventors:
UMEMOTO NAOYUKI (JP)
OTSUKA MASAKO (JP)
OTSUKA MASAKO (JP)
Application Number:
PCT/JP2013/058418
Publication Date:
September 26, 2013
Filing Date:
March 22, 2013
Export Citation:
Assignee:
KIRIN HOLDINGS KK (JP)
International Classes:
C12N15/09; A01H1/00; A01H1/06; A01H5/00; C12Q1/68
Domestic Patent References:
| WO2011025011A1 | 2011-03-03 | |||
| WO2008092910A1 | 2008-08-07 | |||
| WO2011025011A1 | 2011-03-03 |
Foreign References:
| JP2012239412A | 2012-12-10 | |||
| JP2012050374A | 2012-03-15 | |||
| JP2012068039A | 2012-04-05 | |||
| US4683195A | 1987-07-28 | |||
| US4683202A | 1987-07-28 | |||
| US4800159A | 1989-01-24 | |||
| US4965188A | 1990-10-23 | |||
| JP2004535960A | 2004-12-02 | |||
| JP2001161373A | 2001-06-19 | |||
| JP2011027429A | 2011-02-10 |
Other References:
DATABASE GENBANK [online] 22 May 2013 (2013-05-22), "SOLANUM TUBEROSUM MRNA FOR STEROL REDUCTASE (DWF1/DIMINUTO GENE)", XP003033889, Database accession no. FN995650.1
KOZUKUE,N. ET AL.: "Tracer studies on the incorporation of [2-14C]-DL-Mevalonate into chlorophylls a and b, a-chanonine, and a-solanine of potateo sprouts", J.AGRIC.FOOD CHEM., vol. 49, 2001, pages 92 - 97, XP055168271
MCCUE,K.F. ET AL.: "Metabolic compensation of steroidal glycoalkaloid biosynthesis in transgenic potato tubers: using reverse genetics to confirm th in vivo enzyme function of a steroidal alkaloid galactosyltransgerase", PLANT SCI., vol. 168, 2005, pages 267 - 273, XP027783424
CHEVY,F. ET AL.: "Limb malformations of rat fetuses exposed to A distal inhibitor of cholesterol biosynthesis", J.LIPID RES., vol. 43, 2002, pages 1192 - 1200, XP055168275
EICH: "Solanaceae and Convolvulaceae: Secondary Metabolite", 2008, SPRINGER
GINZBERG ET AL., POTATO RESEARCH, vol. 52, 2009, pages 1 - 15
NOHARA ET AL., J. NAT. PROD., vol. 73, 2010, pages 1734 - 1741
MCCUE ET AL., PLANT SCI., vol. 168, 2005, pages 267 - 273
MCCUE ET AL., PHYTOCHEMISTRY, vol. 67, 2006, pages 1590 - 1597
MCCUE ET AL., PHYTOCHEMISTRY, vol. 68, 2007, pages 327 - 334
ARNQVIST ET AL., PLANT PHYSIOL., vol. 131, 2003, pages 1792 - 1799
CHOE ET AL., PLANT PHYSIOL., vol. 119, 1999, pages 897 - 907
KLAHRE ET AL., PLANT CELL, vol. 10, 1998, pages 1677 - 1690
HEFTMANN, PHYTOCHEMISTRY, vol. 22, 1983, pages 1843 - 1860
ECKART EICH: "Solanaceae and Convolvulaceae: Secondary Metabolite", 2008, SPRINGER, pages: 368 - 373
KANEKO ET AL., PHYTOCHEMISTRY, vol. 16, 1977, pages 791 - 793
P. M. DEWICK: "Medicinal Natural Product", 2009, JOHN WILEY & SONS
BAKER ET AL., BIOCHEM. BIOPHYS. RES. COMMUN., vol. 213, 1995, pages 154 - 160
SUNG ET AL., BIO. PHARM. BULL., vol. 18, 1995, pages 1459 - 1461
COREY ET AL., PROC. NATL. ACAD. SCI., U.S.A., vol. 91, 1994, pages 2211 - 2215
SHI ET AL., PROC. NATL. ACAD. SCI., U.S.A., vol. 91, 1994, pages 7370 - 7374
COREY ET AL., PROC. NATL. ACAD. SCI., U.S.A., vol. 90, 1993, pages 11628 - 11632
F. M. AUSBEL ET AL.: "Short Protocols in Molecular Biology", 1995, JOHN WILEY & SONS
SCIENCE, vol. 333, 2011, pages 307
TILL ET AL., GENOME RES, vol. 13, 2003, pages 524 - 530
"Jagaimo Jiten", 2012, ZENKOKU NOSON KYOIKU KYOKAI, CO., LTD.
"Handbook of Potato Production, Improvement, and Postharvest Management", 2006, HAWORTH PRESS INC, pages: 77 - 108
NATURE, vol. 475, 2011, pages 189 - 95
"Genomu reberu no iden kaiseki (Genome-level genetic analysis) MAP and QTL", YASUO UKAI, 2001
AM. J, HUM, GENET., vol. 69, 2001, pages 685 - 94
LARKIN ET AL.: "Clustal W and Clustal X, Version 2.0", BIOINFORMATICS, vol. 23, 2007, pages 2947 - 2948
XU ET AL., NATURE, vol. 475, 2011, pages 189 - 197
CHUANG; MEYEROWITZ, PROC. NATL. ACAD. SCI,, U.S.A., vol. 97, 2000, pages 4985 - 90
WESLEY ET AL., PLANT J., vol. 27, 2001, pages 581 - 90
GELVIN; SCHILPEROOR: "Plant Molecular Biology Manual", vol. C2, 1994, KLUWER ACADEMIC PUBLISHERS
MURASHIGE; SKOOG, PHYSIOL. PLANT., vol. 15, 1962, pages 473 - 497
MONMA: "Shokubutsu Soshiki Baiyo", PLANT TISSUE CULTURE, vol. 7, 1990, pages 57 - 63
SUN ET AL., PLANT CELL PHYSIOL., vol. 47, 2006, pages 426 - 431
RADIATION RESEARCH, vol. 154, 2000, pages 485 - 496
NATURE PROTOCOL, vol. 1, pages 2465 - 2477
See also references of EP 2829607A4
KOZUKUE,N. ET AL.: "Tracer studies on the incorporation of [2-14C]-DL-Mevalonate into chlorophylls a and b, a-chanonine, and a-solanine of potateo sprouts", J.AGRIC.FOOD CHEM., vol. 49, 2001, pages 92 - 97, XP055168271
MCCUE,K.F. ET AL.: "Metabolic compensation of steroidal glycoalkaloid biosynthesis in transgenic potato tubers: using reverse genetics to confirm th in vivo enzyme function of a steroidal alkaloid galactosyltransgerase", PLANT SCI., vol. 168, 2005, pages 267 - 273, XP027783424
CHEVY,F. ET AL.: "Limb malformations of rat fetuses exposed to A distal inhibitor of cholesterol biosynthesis", J.LIPID RES., vol. 43, 2002, pages 1192 - 1200, XP055168275
EICH: "Solanaceae and Convolvulaceae: Secondary Metabolite", 2008, SPRINGER
GINZBERG ET AL., POTATO RESEARCH, vol. 52, 2009, pages 1 - 15
NOHARA ET AL., J. NAT. PROD., vol. 73, 2010, pages 1734 - 1741
MCCUE ET AL., PLANT SCI., vol. 168, 2005, pages 267 - 273
MCCUE ET AL., PHYTOCHEMISTRY, vol. 67, 2006, pages 1590 - 1597
MCCUE ET AL., PHYTOCHEMISTRY, vol. 68, 2007, pages 327 - 334
ARNQVIST ET AL., PLANT PHYSIOL., vol. 131, 2003, pages 1792 - 1799
CHOE ET AL., PLANT PHYSIOL., vol. 119, 1999, pages 897 - 907
KLAHRE ET AL., PLANT CELL, vol. 10, 1998, pages 1677 - 1690
HEFTMANN, PHYTOCHEMISTRY, vol. 22, 1983, pages 1843 - 1860
ECKART EICH: "Solanaceae and Convolvulaceae: Secondary Metabolite", 2008, SPRINGER, pages: 368 - 373
KANEKO ET AL., PHYTOCHEMISTRY, vol. 16, 1977, pages 791 - 793
P. M. DEWICK: "Medicinal Natural Product", 2009, JOHN WILEY & SONS
BAKER ET AL., BIOCHEM. BIOPHYS. RES. COMMUN., vol. 213, 1995, pages 154 - 160
SUNG ET AL., BIO. PHARM. BULL., vol. 18, 1995, pages 1459 - 1461
COREY ET AL., PROC. NATL. ACAD. SCI., U.S.A., vol. 91, 1994, pages 2211 - 2215
SHI ET AL., PROC. NATL. ACAD. SCI., U.S.A., vol. 91, 1994, pages 7370 - 7374
COREY ET AL., PROC. NATL. ACAD. SCI., U.S.A., vol. 90, 1993, pages 11628 - 11632
F. M. AUSBEL ET AL.: "Short Protocols in Molecular Biology", 1995, JOHN WILEY & SONS
SCIENCE, vol. 333, 2011, pages 307
TILL ET AL., GENOME RES, vol. 13, 2003, pages 524 - 530
"Jagaimo Jiten", 2012, ZENKOKU NOSON KYOIKU KYOKAI, CO., LTD.
"Handbook of Potato Production, Improvement, and Postharvest Management", 2006, HAWORTH PRESS INC, pages: 77 - 108
NATURE, vol. 475, 2011, pages 189 - 95
"Genomu reberu no iden kaiseki (Genome-level genetic analysis) MAP and QTL", YASUO UKAI, 2001
AM. J, HUM, GENET., vol. 69, 2001, pages 685 - 94
LARKIN ET AL.: "Clustal W and Clustal X, Version 2.0", BIOINFORMATICS, vol. 23, 2007, pages 2947 - 2948
XU ET AL., NATURE, vol. 475, 2011, pages 189 - 197
CHUANG; MEYEROWITZ, PROC. NATL. ACAD. SCI,, U.S.A., vol. 97, 2000, pages 4985 - 90
WESLEY ET AL., PLANT J., vol. 27, 2001, pages 581 - 90
GELVIN; SCHILPEROOR: "Plant Molecular Biology Manual", vol. C2, 1994, KLUWER ACADEMIC PUBLISHERS
MURASHIGE; SKOOG, PHYSIOL. PLANT., vol. 15, 1962, pages 473 - 497
MONMA: "Shokubutsu Soshiki Baiyo", PLANT TISSUE CULTURE, vol. 7, 1990, pages 57 - 63
SUN ET AL., PLANT CELL PHYSIOL., vol. 47, 2006, pages 426 - 431
RADIATION RESEARCH, vol. 154, 2000, pages 485 - 496
NATURE PROTOCOL, vol. 1, pages 2465 - 2477
See also references of EP 2829607A4
Attorney, Agent or Firm:
HIRAKI Yusuke et al. (JP)
Yusuke Hiraki (JP)
Yusuke Hiraki (JP)
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