Title:
GENE EXPRESSION PROFILING
Document Type and Number:
WIPO Patent Application WO2003008538
Kind Code:
A3
Abstract:
Disclosed are methods and compositions for manipulating and detecting nucleic acids. The method generally involves association of a rolling circle replication primer with a cDNA strand. Preferred forms of the methods involve replicating one or more amplification target circles to produce one or more tandem sequence DNAs. Such replication is referred to as rolling circle replication. Preferably, each tandem sequence DNA is coupled to a rolling circle replication primer and the rolling circle replication primer is associated with a cDNA strand. In some embodiments the rolling circle replication primer comprises a capture tag and the association occurs via the capture tag. In some embodiments the cDNA strand is hybridized to a capture probe. Preferably, the cDNA strand comprises an RT primer, wherein the cDNA strand is produced by reverse transcribing a nucleic acid sample with the RT primer.
Inventors:
NALLUR GIRISH N
LUO CHENGHUA
CHOWDHURY KAJAL
PINARD ROBERT
LUO CHENGHUA
CHOWDHURY KAJAL
PINARD ROBERT
Application Number:
PCT/US2002/015045
Publication Date:
April 10, 2003
Filing Date:
May 10, 2002
Export Citation:
Assignee:
MOLECULAR STAGING INC (US)
International Classes:
C12Q1/68; (IPC1-7): A61K38/18; C07H21/00; C07H21/02; C07H21/04; C07K14/475; C12N15/52; C12P19/34; C12Q1/68; G01N33/574
Foreign References:
| US6143495A | 2000-11-07 | |||
| US5854033A | 1998-12-29 | |||
| US6017703A | 2000-01-25 | |||
| US6057105A | 2000-05-02 | |||
| US6132728A | 2000-10-17 | |||
| US5886329A | 1999-03-23 | |||
| US5821084A | 1998-10-13 | |||
| US6248535B1 | 2001-06-19 |
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