Title:
GENE EXPRESSION PROMOTOR FOR BIFIDOBACTERIUM GENUS BACTERIA
Document Type and Number:
WIPO Patent Application WO/2018/062225
Kind Code:
A1
Abstract:
The present invention addresses the problem of providing a promotor capable of efficiently expressing genes, in Bifidobacterium genus bacteria. In the present invention, a promotor including a TTGNNN motif and a TATANT motif located downstream of the TTGNNN motif, preferably a promotor including a TTGTGC motif and a TATAAT motif located 11 or 17 bases downstream from the TTGTGC motif, is used as a gene expression promotor for Bifidobacterium genus bacteria.
Inventors:
SUZUKI TOHRU (JP)
KATAOKA SHIRO (JP)
KOZAKAI TOMOYA (JP)
KATAOKA SHIRO (JP)
KOZAKAI TOMOYA (JP)
Application Number:
PCT/JP2017/034854
Publication Date:
April 05, 2018
Filing Date:
September 27, 2017
Export Citation:
Assignee:
ANAEROPHARMA SCIENCE INC (JP)
International Classes:
C12N15/09; C12N1/21; C12N15/11
Domestic Patent References:
| WO2016117508A1 | 2016-07-28 |
Foreign References:
| JPH08500739A | 1996-01-30 |
Other References:
KOZAKAI, TOMOYA ET AL.: "Analysis of the promoter of bifidobacterium), non-official translation (Abstracts of Annual Conference of the Japan Society for Bioscience", BIOTECHNOLOGY AND AGROCHEMISTRY, 5 March 2016 (2016-03-05)
WU, S. M. ET AL.: "Enhanced production of recombinant nattokinase in bacillus subtilis by promoter optimization", WORLD J. MICROBIOL. BIOTECHNOL., vol. 27, 2011, pages 99 - 106, XP019892544
INOUYE, S. ET AL.: "Up-promoter mutations in the lpp gene of escherichia coli", NUCLEIC ACIDS RES., vol. 13, no. 9, 1985, pages 3101 - 3110, XP001316824
AOYAMA, T. ET AL.: "Essential structure of E. coli promoter: effect of spacer length between the two consensus sequences on promoter function", NUCLEIC ACIDS RES., vol. 11, no. 17, 1983, pages 5855 - 5864, XP001316838
TRIEU-CUOT, P. ET AL.: "In vivo transfer of genetic information between gram-positive and gram-negative bacteria", EMBO J., vol. 4, no. 13A, 1985, pages 3583 - 3587, XP001313521
ZAIDE, G. ET AL.: "Identification and characterization of novel and potent transcription promoters of Francisella tularensis", APPL. ENVIRON. MICROBIOL., vol. 77, no. 5, 2011, pages 1608 - 1618, XP055498593
SUBUDHI, S. ET AL.: "Isolation and functional characterization of Spirulina D6D gene promoter: role of a putative GntR transcription factor in transcriptional regulation of D6D gene expression", BIOCHEM. BIOPHYS. RES. COMMUN., vol. 365, 2008, pages 643 - 649, XP022384846
KOZAKAI, TOMOYA ET AL., ANALYSIS OF THE PROMOTER OF BIFIDOBACTERIUM, ABSTRACTS OF THE 68TH SBJ ANNUAL MEETING, 25 August 2016 (2016-08-25)
WU, S. M. ET AL.: "Enhanced production of recombinant nattokinase in bacillus subtilis by promoter optimization", WORLD J. MICROBIOL. BIOTECHNOL., vol. 27, 2011, pages 99 - 106, XP019892544
INOUYE, S. ET AL.: "Up-promoter mutations in the lpp gene of escherichia coli", NUCLEIC ACIDS RES., vol. 13, no. 9, 1985, pages 3101 - 3110, XP001316824
AOYAMA, T. ET AL.: "Essential structure of E. coli promoter: effect of spacer length between the two consensus sequences on promoter function", NUCLEIC ACIDS RES., vol. 11, no. 17, 1983, pages 5855 - 5864, XP001316838
TRIEU-CUOT, P. ET AL.: "In vivo transfer of genetic information between gram-positive and gram-negative bacteria", EMBO J., vol. 4, no. 13A, 1985, pages 3583 - 3587, XP001313521
ZAIDE, G. ET AL.: "Identification and characterization of novel and potent transcription promoters of Francisella tularensis", APPL. ENVIRON. MICROBIOL., vol. 77, no. 5, 2011, pages 1608 - 1618, XP055498593
SUBUDHI, S. ET AL.: "Isolation and functional characterization of Spirulina D6D gene promoter: role of a putative GntR transcription factor in transcriptional regulation of D6D gene expression", BIOCHEM. BIOPHYS. RES. COMMUN., vol. 365, 2008, pages 643 - 649, XP022384846
KOZAKAI, TOMOYA ET AL., ANALYSIS OF THE PROMOTER OF BIFIDOBACTERIUM, ABSTRACTS OF THE 68TH SBJ ANNUAL MEETING, 25 August 2016 (2016-08-25)
Attorney, Agent or Firm:
HIROTA, Masanori (JP)
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