Title:
GENE EXPRESSION SWITCH BASED ON RED LIGHT AND FAR-RED LIGHT REGULATION, AND CONSTRUCTION METHOD AND APPLICATION THEREOF
Document Type and Number:
WIPO Patent Application WO/2021/139596
Kind Code:
A1
Abstract:
Provided are a gene expression switch/system (REDMAP system) based on red light and far-red light regulation for mammalian cells, comprising a photoreceptor and an effector. The REDMAP system has the capabilities of simple design, small modules, high-efficiency induction, and rapid and sensitive transcription expression regulation. The REDMAP system can efficiently and accurately regulate transgenic expression of mammalian cells and a signaling pathway based on protein-protein interaction by means of red light and far-red light, and has the characteristics of non-toxicity, high efficiency, spatio-temporal specificity, reversible induction of gene expression and the like. The REDMAP system can accurately regulate expression of a target gene in vivo, facilitates long-term drug delivery of adeno-associated viruses (AAV), can be used for regulating insulin expression, can be used for various biological and pre-transformation application research, such as regulation of a cell signaling pathway, epigenetic engineering, and cell therapy based on drug delivery, and has potential application value in the fields of basic biological research, mammalian gene engineering, and regenerative medicine.
Inventors:
YE HAIFENG (CN)
ZHOU YANG (CN)
KONG DEQIANG (CN)
WANG XINYI (CN)
ZHOU YANG (CN)
KONG DEQIANG (CN)
WANG XINYI (CN)
Application Number:
PCT/CN2020/142005
Publication Date:
July 15, 2021
Filing Date:
December 31, 2020
Export Citation:
Assignee:
UNIV EAST CHINA NORMAL (CN)
International Classes:
C12N15/85; C12M1/36; C12M1/42; C12N13/00
Foreign References:
| EP2674497A1 | 2013-12-18 | |||
| EP2674497A1 | 2013-12-18 | |||
| CN107177499A | 2017-09-19 |
Other References:
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SILVANA KONERMANN, MARK D. BRIGHAM, ALEXANDRO E. TREVINO, JULIA JOUNG, OMAR O. ABUDAYYEH, CLEA BARCENA, PATRICK D. HSU, NAOMI HABI: "Genome-scale transcriptional activation by an engineered CRISPR-Cas9 complex", NATURE, MACMILLAN JOURNALS LTD., ETC., LONDON, vol. 517, no. 7536, 1 January 2015 (2015-01-01), London, pages 583 - 588, XP055585957, ISSN: 0028-0836, DOI: 10.1038/nature14136
JIAWEI SHAO, SHUAI XUE, GUILING YU, YUANHUAN YU, XUEPING YANG, YU BAI, SUCHENG ZHU, LINFENG YANG, JIANLI YIN, YIDAN WANG, SHUYONG : "Smartphone-controlled optogenetically engineered cells enable semiautomatic glucose homeostasis in diabetic mice", SCIENCE TRANSLATIONAL MEDICINE, AMERICAN ASSOCIATION FOR THE ADVANCEMENT OF SCIENCE (A A A S), US, vol. 9, no. 387, 26 April 2017 (2017-04-26), US, pages eaal2298, XP055613568, ISSN: 1946-6234, DOI: 10.1126/scitranslmed.aal2298
JULIA RAUSENBERGER; ANKE TSCHEUSCHLER; WIEBKE NORDMEIER; FLORIAN WST; JENS TIMMER; EBERHARD SCHFER; CHRISTIAN FLECK; ANDREAS HILTB: "Photoconversion and Nuclear Trafficking Cycles Determine Phytochrome A's Response Profile to Far-Red Light", CELL, ELSEVIER, AMSTERDAM NL, vol. 146, no. 5, 13 July 2011 (2011-07-13), Amsterdam NL, pages 813 - 825, XP028278963, ISSN: 0092-8674, DOI: 10.1016/j.cell.2011.07.023
TOETTCHER JARED�E.; WEINER ORION�D.; LIM WENDELL�A.: "Using Optogenetics to Interrogate the Dynamic Control of Signal Transmission by the Ras/Erk Module", CELL, ELSEVIER, AMSTERDAM NL, vol. 155, no. 6, 5 December 2013 (2013-12-05), Amsterdam NL, pages 1422 - 1434, XP028805086, ISSN: 0092-8674, DOI: 10.1016/j.cell.2013.11.004
SILVANA KONERMANN, MARK D. BRIGHAM, ALEXANDRO E. TREVINO, JULIA JOUNG, OMAR O. ABUDAYYEH, CLEA BARCENA, PATRICK D. HSU, NAOMI HABI: "Genome-scale transcriptional activation by an engineered CRISPR-Cas9 complex", NATURE, MACMILLAN JOURNALS LTD., ETC., LONDON, vol. 517, no. 7536, 1 January 2015 (2015-01-01), London, pages 583 - 588, XP055585957, ISSN: 0028-0836, DOI: 10.1038/nature14136
JIAWEI SHAO, SHUAI XUE, GUILING YU, YUANHUAN YU, XUEPING YANG, YU BAI, SUCHENG ZHU, LINFENG YANG, JIANLI YIN, YIDAN WANG, SHUYONG : "Smartphone-controlled optogenetically engineered cells enable semiautomatic glucose homeostasis in diabetic mice", SCIENCE TRANSLATIONAL MEDICINE, AMERICAN ASSOCIATION FOR THE ADVANCEMENT OF SCIENCE (A A A S), US, vol. 9, no. 387, 26 April 2017 (2017-04-26), US, pages eaal2298, XP055613568, ISSN: 1946-6234, DOI: 10.1126/scitranslmed.aal2298
JULIA RAUSENBERGER; ANKE TSCHEUSCHLER; WIEBKE NORDMEIER; FLORIAN WST; JENS TIMMER; EBERHARD SCHFER; CHRISTIAN FLECK; ANDREAS HILTB: "Photoconversion and Nuclear Trafficking Cycles Determine Phytochrome A's Response Profile to Far-Red Light", CELL, ELSEVIER, AMSTERDAM NL, vol. 146, no. 5, 13 July 2011 (2011-07-13), Amsterdam NL, pages 813 - 825, XP028278963, ISSN: 0092-8674, DOI: 10.1016/j.cell.2011.07.023
Attorney, Agent or Firm:
MSJLAW OFFICE (CN)
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