Title:
GENE, MICROORGANISM, CONVERSION METHOD, AND PRODUCTION METHOD
Document Type and Number:
WIPO Patent Application WO/2014/046178
Kind Code:
A1
Abstract:
The present invention is a gene set forth in one of the belowmentioned (a) to (c), wherein by means of a combination of genes coding for enoyl-CoA hydratase, it is possible to impart to a microorganism or a culture of the microorganism the ability to convert 3-hydroxybutyryl-CoA to 4-hydroxybutyryl CoA, or 4-hydroxybutyryl-CoA to 3-hydroxybutyryl-CoA. (a) A gene having the base sequence in sequence number 1. (b) A gene having a base sequence that results from one or more bases being deleted, substituted, or inserted with respect to the base sequence of sequence number 1, and that is at last 90% identical to the base sequence of sequence number 1. (c) A gene that hybridizes under stringent conditions with a gene having a base sequence complementary to a gene having the base sequence set forth in sequence number 1.
Inventors:
AOKI HIROBUMI (JP)
KOKIDO YUZURU (JP)
YONEDA TADASHI (JP)
KOKIDO YUZURU (JP)
YONEDA TADASHI (JP)
Application Number:
PCT/JP2013/075296
Publication Date:
March 27, 2014
Filing Date:
September 19, 2013
Export Citation:
Assignee:
SHOWA DENKO KK (JP)
International Classes:
C12N15/09; C12N1/19; C12N1/21; C12N9/02; C12N9/88; C12P7/18
Domestic Patent References:
| WO2008115840A2 | 2008-09-25 | |||
| WO2010127319A2 | 2010-11-04 |
Foreign References:
| JP2011509691A | 2011-03-31 | |||
| JP2002199890A | 2002-07-16 | |||
| JP4380704B2 | 2009-12-09 |
Other References:
DATABASE GENBANK 13 May 2008 (2008-05-13), GERHARDT A. ET AL.: "Definition: Clostridium aminobutyricum abfD gene, abfT gene and abfH gene (partial) for dehydration of 4- hydroxybutyrate.", retrieved from http://www.ncbi.nlm.nih.gov/nuccore/ aj250267 accession no. J250267
GERHARDT A. ET AL.: "Fermentation of 4- aminobutyrate by Clostridium aminobutyricum: cloning of two genes involved in the formation and dehydration of 4-hydroxybutyryl-CoA.", ARCH. MICROBIOL., vol. 174, no. 3, 17 September 2000 (2000-09-17), pages 189 - 99
SCHERF U. ET AL.: "Purification and properties of an iron-sulfur and FAD-containing 4- hydroxybutyryl-CoA dehydratase/vinylacetyl- CoA delta 3-delta 2-isomerase from Clostridium aminobutyricum.", EUR.J.BIOCHEM., vol. 215, no. 2, 15 July 1993 (1993-07-15), pages 421 - 9
MAKOFF A.J. ET AL.: "Expression of tetanus toxin fragment C in E. coli: high level expression by removing rare codons.", NUCLEIC ACIDS RES., vol. 17, no. 24, 25 December 1989 (1989-12-25), pages 10191 - 202
FRANCIS D.M. ET AL.: "Strategies to optimize protein expression in E. coli.", CURR.PROTOC. PROTEIN SCI., August 2010 (2010-08-01), pages 1 - 29
FONTAINE L. ET AL.: "Molecular characterization and transcriptional analysis of adhE2, the gene encoding the NADH-dependent aldehyde/alcohol dehydrogenase responsible for butanol production in alcohologenic cultures of Clostridium acetobutylicum ATCC 824.", J. BACTERIOL., vol. 184, no. 3, 18 February 2002 (2002-02-18), pages 821 - 30
TOMIZAWA S. ET AL.: "Preparative synthesis of Poly[(R)-3-hydroxybutyrate] monomer for enzymatic cell-free polymerization.", POLYMER JOURNAL, vol. 44, no. 9, 21 March 2012 (2012-03-21), pages 982 - 5
See also references of EP 2899273A4
HARRY YIM ET AL.: "Metabolic engineering of Escherichia coli for direct production of 1,4-butanediol", NATURE CHEMICAL BIOLOGY, vol. 7, 2011, pages 445 - 452
JOSEPH SAMBROOK; DAVID W. RUSSELL: "Molecular Cloning - A LABORATORY MANUAL", COLD SPRING HARBOR LABORATORY PRESS
ARCHIVES OF MICROBIOLOGY, vol. 174, no. 3, 2000, pages 189 - 199
"Molecular analysis of the anaerobic succinate degradation pathway in Clostridium kluyveri", JOURNAL OF BACTERIOLOGY, vol. 178, no. 3, 1996, pages 871 - 880
FRIEDRICH P; DARLEY DJ; GOLDING BT; BUCKEL W.: "The complete stereochemistry of the enzymatic dehydration of 4-hydroxybutyryl coenzyme A to crotonyl coenzyme. A.", ANGEWANDTE CHEMIE INTERNATIONAL EDITION, vol. 47, no. 17, 2008, pages 3254 - 3257
MOSKOWITZ GJ; MERRICK JM: "Metabolism of poly-beta-hydroxybutyrate. II. Enzymatic synthesis of D-(-)-beta hydroxybutyryl coenzyme A by an enoyl hydrase from Rhodospirillum rubrum", JOURNAL BIOCHEMISTRY, vol. 8, 1969, pages 2748 - 2755
"Purification, properties, and kinetic mechanism of coenzyme A-linked aldehyde dehydrogenase from Clostridium kluyveri", JOURNAL ARCH. BIOCHEM. BIOPHYS., vol. 203, 1980, pages 663 - 75
"Molecular Characterization and Transcriptional Analysis of adhE2, the Gene Encoding the NADH-Dependent Aldehyde/Alcohol Dehydrogenase Responsible for Butanol Production in Alcohologenic Cultures of Clostridium acetobutylicum ATCC 824.", JOURNAL OF BACTERIOLOGY, vol. 184, no. 3, 2002, pages 821 - 830
GERHARDT A. ET AL.: "Fermentation of 4- aminobutyrate by Clostridium aminobutyricum: cloning of two genes involved in the formation and dehydration of 4-hydroxybutyryl-CoA.", ARCH. MICROBIOL., vol. 174, no. 3, 17 September 2000 (2000-09-17), pages 189 - 99
SCHERF U. ET AL.: "Purification and properties of an iron-sulfur and FAD-containing 4- hydroxybutyryl-CoA dehydratase/vinylacetyl- CoA delta 3-delta 2-isomerase from Clostridium aminobutyricum.", EUR.J.BIOCHEM., vol. 215, no. 2, 15 July 1993 (1993-07-15), pages 421 - 9
MAKOFF A.J. ET AL.: "Expression of tetanus toxin fragment C in E. coli: high level expression by removing rare codons.", NUCLEIC ACIDS RES., vol. 17, no. 24, 25 December 1989 (1989-12-25), pages 10191 - 202
FRANCIS D.M. ET AL.: "Strategies to optimize protein expression in E. coli.", CURR.PROTOC. PROTEIN SCI., August 2010 (2010-08-01), pages 1 - 29
FONTAINE L. ET AL.: "Molecular characterization and transcriptional analysis of adhE2, the gene encoding the NADH-dependent aldehyde/alcohol dehydrogenase responsible for butanol production in alcohologenic cultures of Clostridium acetobutylicum ATCC 824.", J. BACTERIOL., vol. 184, no. 3, 18 February 2002 (2002-02-18), pages 821 - 30
TOMIZAWA S. ET AL.: "Preparative synthesis of Poly[(R)-3-hydroxybutyrate] monomer for enzymatic cell-free polymerization.", POLYMER JOURNAL, vol. 44, no. 9, 21 March 2012 (2012-03-21), pages 982 - 5
See also references of EP 2899273A4
HARRY YIM ET AL.: "Metabolic engineering of Escherichia coli for direct production of 1,4-butanediol", NATURE CHEMICAL BIOLOGY, vol. 7, 2011, pages 445 - 452
JOSEPH SAMBROOK; DAVID W. RUSSELL: "Molecular Cloning - A LABORATORY MANUAL", COLD SPRING HARBOR LABORATORY PRESS
ARCHIVES OF MICROBIOLOGY, vol. 174, no. 3, 2000, pages 189 - 199
"Molecular analysis of the anaerobic succinate degradation pathway in Clostridium kluyveri", JOURNAL OF BACTERIOLOGY, vol. 178, no. 3, 1996, pages 871 - 880
FRIEDRICH P; DARLEY DJ; GOLDING BT; BUCKEL W.: "The complete stereochemistry of the enzymatic dehydration of 4-hydroxybutyryl coenzyme A to crotonyl coenzyme. A.", ANGEWANDTE CHEMIE INTERNATIONAL EDITION, vol. 47, no. 17, 2008, pages 3254 - 3257
MOSKOWITZ GJ; MERRICK JM: "Metabolism of poly-beta-hydroxybutyrate. II. Enzymatic synthesis of D-(-)-beta hydroxybutyryl coenzyme A by an enoyl hydrase from Rhodospirillum rubrum", JOURNAL BIOCHEMISTRY, vol. 8, 1969, pages 2748 - 2755
"Purification, properties, and kinetic mechanism of coenzyme A-linked aldehyde dehydrogenase from Clostridium kluyveri", JOURNAL ARCH. BIOCHEM. BIOPHYS., vol. 203, 1980, pages 663 - 75
"Molecular Characterization and Transcriptional Analysis of adhE2, the Gene Encoding the NADH-Dependent Aldehyde/Alcohol Dehydrogenase Responsible for Butanol Production in Alcohologenic Cultures of Clostridium acetobutylicum ATCC 824.", JOURNAL OF BACTERIOLOGY, vol. 184, no. 3, 2002, pages 821 - 830
Attorney, Agent or Firm:
ITOH, Tadashige et al. (JP)
Tadashige Ito (JP)
Tadashige Ito (JP)
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