Title:
GENE-MODIFIED MICROORGANISM FOR PRODUCING 3-HYDROXYADIPIC ACID, α-HYDROMUCONIC ACID AND/OR ADIPIC ACID, AND PRODUCTION METHOD FOR SAID CHEMICAL PRODUCTS
Document Type and Number:
WIPO Patent Application WO/2019/107516
Kind Code:
A1
Abstract:
Disclosed is a gene-modified microorganism wherein nucleic acids have been introduced which code for an enzyme catalyzing a reaction which reduces 3-oxoadipyl-CoA to 3-hydroxyadipyl-CoA. In the gene-modified microorganism, either: nucleic acids have been introduced which code for polypeptides described in (a)-(c) below; or, the expression of the polypeptides is enhanced. (a) A polypeptide formed from any amino acid sequence from SEQ ID NO:1-6 and 213, (b) a polypeptide comprising any of the amino acid sequences from SEQ ID NO:1-6 and 213 in which at least one amino acid has been substituted, deleted, inserted and/or added, said polypeptide having enzyme activity catalyzing a reaction which reduces 3-oxoadipyl-CoA to generate 3-hydroxyadipyl-CoA, and (c) a polypeptide having at least 70% sequence identity with any of the amino acid sequences from SEQ ID NO:1-6 and 213, said polypeptide having activity which reduces 3-oxoadipyl-CoA to generate 3-hydroxyadipyl-CoA.
Inventors:
ISOBE KYOHEI (JP)
KAWAMURA KENJI (JP)
YAMADA KATSUSHIGE (JP)
KAWAMURA KENJI (JP)
YAMADA KATSUSHIGE (JP)
Application Number:
PCT/JP2018/044080
Publication Date:
June 06, 2019
Filing Date:
November 29, 2018
Export Citation:
Assignee:
TORAY INDUSTRIES (JP)
International Classes:
C12N9/04; C12N1/21; C12P7/42; C12P7/44; C12N15/53
Domestic Patent References:
| WO2016106367A1 | 2016-06-30 | |||
| WO2017209103A1 | 2017-12-07 | |||
| WO2017099209A1 | 2017-06-15 | |||
| WO2016199858A1 | 2016-12-15 | |||
| WO2016199856A1 | 2016-12-15 | |||
| WO2016199858A1 | 2016-12-15 |
Foreign References:
| JP2011515111A | 2011-05-19 | |||
| JP2013535203A | 2013-09-12 | |||
| JP2013535203A | 2013-09-12 | |||
| US20110124911A1 | 2011-05-26 | |||
| JP2011515111A | 2011-05-19 |
Other References:
DATABASE PROTEIN [online] 28 February 2017 (2017-02-28), ANONYMOUS: "3-hydroxybutyryl-CoA dehydrogenase [Serratia marcescens)", XP055615559, retrieved from NCBI Database accession no. ALL37524
DATABASE PROTEIN [online] 7 August 2014 (2014-08-07), ANONYMOUS: "3-hydroxybutyryl-CoA dehydrogenase [Serratia nematodiphila DZ 0503SBS1", XP055615561, retrieved from NCBI Database accession no. KFF90258
DATABASE PROTEIN [online] 30 January 2014 (2014-01-30), ANONYMOUS: ", 3-hydroxybutyryl-CoA dehydrogenase [Serratia plymuthica S13", XP055615562, retrieved from NCBI Database accession no. AGP43956
DATABASE PROTEIN [online] 28 January 2014 (2014-01-28), ANONYMOUS: "3-hydroxybutyryl-CoA dehydrogenase [Serratia proteamaculans 568)", XP055615565, retrieved from NCBI Database accession no. ABV40935
DATABASE PROTEIN [online] 18 July 2017 (2017-07-18), ANONYMOUS: "3-hydroxybutyryl-CoA dehydrogenase [Serratia marcescens)", XP055615570, retrieved from NCBI Database accession no. ASM11476
DATABASE PROTEIN [online] 14 April 2017 (2017-04-14), ANONYMOUS: "3-hydroxybutyryl-CoA dehydrogenase [Serratia proteamaculans)", XP055615574, retrieved from NCBI Database accession no. SMB31239
DATABASE PROTEIN [online] 11 February 2016 (2016-02-11), ANONYMOUS: "3-hydroxybutyry 1-CoA dehydrogenase [Serratia liquefaciens)", XP055615577, retrieved from NCBI Database accession no. AMG98270
BIOCHIMIE, vol. 94, no. 2, February 2012 (2012-02-01), pages 471 - 8
"NCBI", Database accession no. NC _ 000913.3
"NCBl", Database accession no. 1046612
J APPL MICROBIOL., vol. 119, no. 4, October 2015 (2015-10-01), pages 1057 - 63
"UniProtKB", Database accession no. Q5EU90
ME KOVACH, GENE, vol. 166, 1995, pages 175 - 176
NM CALVINPC 1 LANAWALT, J. BACTERIOL, vol. 170, 1988, pages 2796 - 2801
PROC NATL ACAD SCI U.S.A., vol. 97, no. 12, 6 June 2000 (2000-06-06), pages 6640 - 6645
BIOSCI BIOTECHNOL BIOCHEM., vol. 71, no. 12, December 2007 (2007-12-01), pages 2905 - 11
JOURNAL OF MOLECULAR BIOLOGY, vol. 53, 1970, pages 159
NM CALVINPC HANAWALT, J. BACTERID, vol. 170, 1988, pages 2796 - 2801
NM CALVINPC HANAWALT, J. BACTCRIOL, vol. 170, 1988, pages 2796 - 2801
KASCHABEK ET AL., J BACTERIOL., vol. 184, no. 1, January 2002 (2002-01-01), pages 207 - 215
DATABASE PROTEIN [online] 7 August 2014 (2014-08-07), ANONYMOUS: "3-hydroxybutyryl-CoA dehydrogenase [Serratia nematodiphila DZ 0503SBS1", XP055615561, retrieved from NCBI Database accession no. KFF90258
DATABASE PROTEIN [online] 30 January 2014 (2014-01-30), ANONYMOUS: ", 3-hydroxybutyryl-CoA dehydrogenase [Serratia plymuthica S13", XP055615562, retrieved from NCBI Database accession no. AGP43956
DATABASE PROTEIN [online] 28 January 2014 (2014-01-28), ANONYMOUS: "3-hydroxybutyryl-CoA dehydrogenase [Serratia proteamaculans 568)", XP055615565, retrieved from NCBI Database accession no. ABV40935
DATABASE PROTEIN [online] 18 July 2017 (2017-07-18), ANONYMOUS: "3-hydroxybutyryl-CoA dehydrogenase [Serratia marcescens)", XP055615570, retrieved from NCBI Database accession no. ASM11476
DATABASE PROTEIN [online] 14 April 2017 (2017-04-14), ANONYMOUS: "3-hydroxybutyryl-CoA dehydrogenase [Serratia proteamaculans)", XP055615574, retrieved from NCBI Database accession no. SMB31239
DATABASE PROTEIN [online] 11 February 2016 (2016-02-11), ANONYMOUS: "3-hydroxybutyry 1-CoA dehydrogenase [Serratia liquefaciens)", XP055615577, retrieved from NCBI Database accession no. AMG98270
BIOCHIMIE, vol. 94, no. 2, February 2012 (2012-02-01), pages 471 - 8
"NCBI", Database accession no. NC _ 000913.3
"NCBl", Database accession no. 1046612
J APPL MICROBIOL., vol. 119, no. 4, October 2015 (2015-10-01), pages 1057 - 63
"UniProtKB", Database accession no. Q5EU90
ME KOVACH, GENE, vol. 166, 1995, pages 175 - 176
NM CALVINPC 1 LANAWALT, J. BACTERIOL, vol. 170, 1988, pages 2796 - 2801
PROC NATL ACAD SCI U.S.A., vol. 97, no. 12, 6 June 2000 (2000-06-06), pages 6640 - 6645
BIOSCI BIOTECHNOL BIOCHEM., vol. 71, no. 12, December 2007 (2007-12-01), pages 2905 - 11
JOURNAL OF MOLECULAR BIOLOGY, vol. 53, 1970, pages 159
NM CALVINPC HANAWALT, J. BACTERID, vol. 170, 1988, pages 2796 - 2801
NM CALVINPC HANAWALT, J. BACTCRIOL, vol. 170, 1988, pages 2796 - 2801
KASCHABEK ET AL., J BACTERIOL., vol. 184, no. 1, January 2002 (2002-01-01), pages 207 - 215
Attorney, Agent or Firm:
TANIGAWA AND PARTNERS, PATENT FIRM (JP)
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