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Title:
GENE MODULE FOR EFFICIENTLY DEGRADING FEATHER AND SYNTHESIZING ARTIFICIAL HEMEPROTEIN, AND APPLICATION THEREOF
Document Type and Number:
WIPO Patent Application WO/2022/007145
Kind Code:
A1
Abstract:
A functional module SyKerMb capable of efficiently degrading poultry feather waste and synthesizing a novel hemeprotein is artificially designed and constructed by using a synthetic biology method. A recombinant vector of the functional module is constructed, and assembled and expressed in chassis microorganism cell Escherichia coli. Experiments prove that engineering strain integrating the transformation function module has the biological synthesis capability of degrading animal feather waste and efficiently synthesizing an artificial hemeprotein, and can be applied in related industrial production of artificial protein preparations and future artificial food.

Inventors:
LIN MIN (CN)
ZHOU ZHENGFU (CN)
YAN YONGLIANG (CN)
ZHANG WEI (CN)
WANG JIN (CN)
LU WEI (CN)
Application Number:
PCT/CN2020/111685
Publication Date:
January 13, 2022
Filing Date:
August 27, 2020
Export Citation:
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Assignee:
BIOTECHNOLOGY RES INSTITUTE CHINESE ACADEMY OF AGRICULTURAL SCIENCES (CN)
International Classes:
C07K14/00; C12N15/11; C12N1/21; C12N15/70; C12P17/18
Foreign References:
CN109161557A2019-01-08
CN109266634A2019-01-25
CN109182310A2019-01-11
CN110305819A2019-10-08
CN104694440A2015-06-10
Other References:
ZHENG PENG;XINZHE MAO;JUAN ZHANG;GUOCHENG DU;JIAN CHEN: "Effective biodegradation of chicken feather waste by co-cultivation of keratinase producing strains", MICROBIAL CELL FACTORIES, vol. 18, no. 84, 1 May 2019 (2019-05-01), pages 1 - 11, XP009533210, ISSN: 1475-2859, DOI: 10.1186/s12934-019-1134-9
MAZOTTO ANA MARIA, DE MELO ANA CRISTINA N., MACRAE ANDREW, ROSADO ALEXANDRE SOARES, PEIXOTO RAQUEL, CEDROLA SABRINA M. L., COURI S: "Biodegradation of feather waste by extracellular keratinases and gelatinases from Bacillus spp", WORLD JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, vol. 27, no. 6, 1 June 2011 (2011-06-01), Dordrecht, pages 1355 - 1365, XP055886567, ISSN: 0959-3993, DOI: 10.1007/s11274-010-0586-1
Attorney, Agent or Firm:
HAIHONG JIACHENG INTELLECTUAL PROPERTY & PARTNERS (CN)
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