GENE PARTICIPATING IN ACETIC ACID TOLERANCE, ACETIC ACID BACTERIUM BRED USING THE GENE, AND PROCESS FOR PRODUCING VINEGAR WITH THE USE OF THE ACETIC ACID BACTERIUM

Title:

GENE PARTICIPATING IN ACETIC ACID TOLERANCE, ACETIC ACID BACTERIUM BRED USING THE GENE, AND PROCESS FOR PRODUCING VINEGAR WITH THE USE OF THE ACETIC ACID BACTERIUM

Document Type and Number:

WIPO Patent Application WO/2003/078635

Kind Code:

A1

Abstract:

It is intended to provide a novel gene participating in the acetic acid tolerance of an acetic acid bacterium; a method of improving the acetic acid tolerance of a microorganism, in particular, an acetic acid bacterium using the gene; and a process for efficiently producing vinegar having an elevated acetic acid concentration with the use of the acetic acid bacterium having the thus improved acetic acid tolerance. A gene enabling an acetic acid bacterium to grow in a medium at such an acetic acid concentration that it cannot grow in usual is obtained from an acetic acid bacterium chromosomal DNA library. Thus, a novel gene having a function of improving the acetic acid tolerance of a practically available acetic acid bacterium belonging to the genus Gluconeacetobacter to a practically usable level is cloned. In a transformant having the above gene transferred into an acetic acid bacterium, the acetic acid tolerance is remarkably improved. When this transformant is cultured under aeration in the presence of ethanol, the growth inductive period can be shortened and the growth speed can be elevated. Moreover, the final acetic acid concentration can be remarkably elevated thereby.

Inventors:

GOTOU HIDETSUGU (JP)
NAKANO SHIGERU (JP)

Application Number:

PCT/JP2003/002946

Publication Date:

September 25, 2003

Filing Date:

March 12, 2003

Export Citation:

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Assignee:

MITSUKAN GROUP CORP (JP)
GOTOU HIDETSUGU (JP)
NAKANO SHIGERU (JP)

International Classes:

C07K14/195; C12J1/04; C12N1/20; C12N1/21; C12N15/54; (IPC1-7): C12N15/54; C12J1/04; C12N1/21; C12N9/10

Foreign References:

EP0332120A2	1989-09-13
US4654306A	1987-03-31
JPS609489A	1985-01-18

Other References: