Title:
GENERATION OF HUMAN CYTOMEGALOVIRUS YEAST ARTIFICIAL CHROMOSOME RECOMBINANTS
Document Type and Number:
WIPO Patent Application WO2002057437
Kind Code:
A3
Abstract:
The present invention relates to a recombinant DNA construct comprising a YAC vector and at least a portion of the HCMV genome. The vector is useful as a basic research tool with which to study CMV biology, or as a vaccine with which to immunize a mammalian host against infection by CMV.
Inventors:
GHAZAL PETER (IE)
HUANG HUANG (US)
HUANG HUANG (US)
Application Number:
PCT/US2001/047943
Publication Date:
August 14, 2003
Filing Date:
October 30, 2001
Export Citation:
Assignee:
SCRIPPS RESEARCH INST (US)
GHAZAL PETER (IE)
HUANG HUANG (US)
GHAZAL PETER (IE)
HUANG HUANG (US)
International Classes:
C12N15/81; C12N15/86; (IPC1-7): C12N15/81; C12N15/86
Domestic Patent References:
| WO1995003400A1 | 1995-02-02 | |||
| WO1999006582A1 | 1999-02-11 | |||
| WO1999043842A1 | 1999-09-02 |
Other References:
KETNER G ET AL: "Efficient manipulation of the human adenovirus genome as an infectious yeast artificial chromosome clone", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA, NATIONAL ACADEMY OF SCIENCE. WASHINGTON, US, vol. 91, 1 June 1994 (1994-06-01), pages 6186 - 6190, XP002086654, ISSN: 0027-8424
MESSERLE ET AL: "CLONING AND MUTAGENESIS OF A HERPESVIRUS GENOME AS AN INFECTIOUS BACTERIAL ARTIFICIAL CHROMOSOME", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA, NATIONAL ACADEMY OF SCIENCE. WASHINGTON, US, vol. 94, December 1997 (1997-12-01), pages 14759 - 14763, XP002104658, ISSN: 0027-8424
HORSBURGH B C ET AL: "ALLEL REPLACEMENT: AN APPLICATION THAT PERMITS RAPID MANIPULATION OF HERPES SIMPLEX VIRUS TYPE 1 GENOMES", GENE THERAPY, MACMILLAN PRESS LTD., BASINGSTOKE, GB, vol. 6, no. 5, May 1999 (1999-05-01), pages 922 - 930, XP001098376, ISSN: 0969-7128
KEMBLE GEORGE ET AL: "Defined large-scale alteration of the human cytomegalovirus genome constructed by cotransfection of overlapping cosmids.", JOURNAL OF VIROLOGY, vol. 70, no. 3, 1996, pages 2044 - 2048, XP002230078, ISSN: 0022-538X
GARCIA-RAMIREZ JOSE J ET AL: "Dominance of virus over host factors in cross-species activation of human cytomegalovirus early gene expression.", JOURNAL OF VIROLOGY, vol. 75, no. 1, January 2001 (2001-01-01), pages 26 - 35, XP002230079, ISSN: 0022-538X
MESSERLE ET AL: "CLONING AND MUTAGENESIS OF A HERPESVIRUS GENOME AS AN INFECTIOUS BACTERIAL ARTIFICIAL CHROMOSOME", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA, NATIONAL ACADEMY OF SCIENCE. WASHINGTON, US, vol. 94, December 1997 (1997-12-01), pages 14759 - 14763, XP002104658, ISSN: 0027-8424
HORSBURGH B C ET AL: "ALLEL REPLACEMENT: AN APPLICATION THAT PERMITS RAPID MANIPULATION OF HERPES SIMPLEX VIRUS TYPE 1 GENOMES", GENE THERAPY, MACMILLAN PRESS LTD., BASINGSTOKE, GB, vol. 6, no. 5, May 1999 (1999-05-01), pages 922 - 930, XP001098376, ISSN: 0969-7128
KEMBLE GEORGE ET AL: "Defined large-scale alteration of the human cytomegalovirus genome constructed by cotransfection of overlapping cosmids.", JOURNAL OF VIROLOGY, vol. 70, no. 3, 1996, pages 2044 - 2048, XP002230078, ISSN: 0022-538X
GARCIA-RAMIREZ JOSE J ET AL: "Dominance of virus over host factors in cross-species activation of human cytomegalovirus early gene expression.", JOURNAL OF VIROLOGY, vol. 75, no. 1, January 2001 (2001-01-01), pages 26 - 35, XP002230079, ISSN: 0022-538X
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