Title:
GENERATION OF INDUCED PLURIPOTENT STEM CELLS WITHOUT THE USE OF VIRAL VECTORS
Document Type and Number:
WIPO Patent Application WO/2010/059806
Kind Code:
A3
Abstract:
Presented herein, generally, are methods for generating reprogrammed mammalian cells, e.g., induced pluripotent stem cells, from differentiated mammalian cells without the use of viral or plasmid vectors. In one aspect, the methods involve contacting a differentiated cell with transducible polypeptides comprising a reprogramming factor polypeptide linked to a cell penetration peptide so that a reprogrammed mammalian cell that exhibits at least one characteristic of pluripotency is generated. Also presented herein are methods for cardiac differentiation of a mammalian cell without the use of viral or plasmid vectors. In one aspect, such methods involve contacting a mammalian cell exhibiting at least one characteristic of pluripotency with a transducible polypeptide, so that cardiac differentiation of the cell occurs.
Inventors:
WHITE ANTHONY J (US)
MAKKAR RAJ R (US)
MARBAN EDUARDO (US)
MAKKAR RAJ R (US)
MARBAN EDUARDO (US)
Application Number:
PCT/US2009/065117
Publication Date:
July 15, 2010
Filing Date:
November 19, 2009
Export Citation:
Assignee:
CEDARS SINAI MEDICAL CENTER (US)
WHITE ANTHONY J (US)
MAKKAR RAJ R (US)
MARBAN EDUARDO (US)
WHITE ANTHONY J (US)
MAKKAR RAJ R (US)
MARBAN EDUARDO (US)
International Classes:
C12N5/07; C12N15/62
Domestic Patent References:
| WO2009032456A2 | 2009-03-12 | |||
| WO2009149956A2 | 2009-12-17 |
Other References:
KWON Y D ET AL: "Cellular Manipulation of Human Embryonic Stem Cells by TAT-PDX1 Protein Transduction", MOLECULAR THERAPY, ACADEMIC PRESS, SAN DIEGO, CA, US, vol. 12, no. 1, 1 July 2005 (2005-07-01), pages 28 - 32, XP004974945, ISSN: 1525-0016
YU JUNYING ET AL: "Induced pluripotent stem cell lines derived from human somatic cells", SCIENCE, AMERICAN ASSOCIATION FOR THE ADVANCEMENT OF SCIENCE, US, vol. 318, no. 5858, 21 December 2007 (2007-12-21), pages 1917 - 1920, XP009105055, ISSN: 1095-9203
TAKAHASHI KAZUTOSHI ET AL: "Induction of pluripotent stem cells from adult human fibroblasts by defined factors", CELL, CELL PRESS, CAMBRIDGE, NA, US, vol. 131, no. 5, 30 November 2007 (2007-11-30), pages 861 - 872, XP002478584, ISSN: 0092-8674
BOSNALI MANAL ET AL: "Generation of transducible versions of transcription factors Oct4 and Sox2", BIOLOGICAL CHEMISTRY, WALTER DE GRUYTER GMBH & CO, BERLIN, DE, vol. 389, no. 7, 1 July 2008 (2008-07-01), pages 851 - 861, XP009118395, ISSN: 1431-6730
ZHOU HONGYAN ET AL: "Generation of Induced Pluripotent Stem Cells Using Recombinant Proteins", CELL STEM CELL, CELL PRESS, US, vol. 4, no. 5, 1 May 2009 (2009-05-01), pages 381 - 384, XP002532629, ISSN: 1934-5909
KIM DOHOON ET AL: "Generation of human induced pluripotent stem cells by direct delivery of reprogramming proteins.", CELL STEM CELL 5 JUN 2009, vol. 4, no. 6, 5 June 2009 (2009-06-05), pages 472 - 476, XP002564507, ISSN: 1875-9777
HENG B C ET AL: "Incorporating protein transduction domains (PTD) within recombinant 'fusion' transcription factors. A novel strategy for directing stem cell differentiation?", BIOMEDICINE AND PHARMACOTHERAPY, ELSEVIER, PARIS, FR, vol. 59, no. 3, 1 April 2005 (2005-04-01), pages 132 - 134, XP004861305, ISSN: 0753-3322
YU JUNYING ET AL: "Induced pluripotent stem cell lines derived from human somatic cells", SCIENCE, AMERICAN ASSOCIATION FOR THE ADVANCEMENT OF SCIENCE, US, vol. 318, no. 5858, 21 December 2007 (2007-12-21), pages 1917 - 1920, XP009105055, ISSN: 1095-9203
TAKAHASHI KAZUTOSHI ET AL: "Induction of pluripotent stem cells from adult human fibroblasts by defined factors", CELL, CELL PRESS, CAMBRIDGE, NA, US, vol. 131, no. 5, 30 November 2007 (2007-11-30), pages 861 - 872, XP002478584, ISSN: 0092-8674
BOSNALI MANAL ET AL: "Generation of transducible versions of transcription factors Oct4 and Sox2", BIOLOGICAL CHEMISTRY, WALTER DE GRUYTER GMBH & CO, BERLIN, DE, vol. 389, no. 7, 1 July 2008 (2008-07-01), pages 851 - 861, XP009118395, ISSN: 1431-6730
ZHOU HONGYAN ET AL: "Generation of Induced Pluripotent Stem Cells Using Recombinant Proteins", CELL STEM CELL, CELL PRESS, US, vol. 4, no. 5, 1 May 2009 (2009-05-01), pages 381 - 384, XP002532629, ISSN: 1934-5909
KIM DOHOON ET AL: "Generation of human induced pluripotent stem cells by direct delivery of reprogramming proteins.", CELL STEM CELL 5 JUN 2009, vol. 4, no. 6, 5 June 2009 (2009-06-05), pages 472 - 476, XP002564507, ISSN: 1875-9777
HENG B C ET AL: "Incorporating protein transduction domains (PTD) within recombinant 'fusion' transcription factors. A novel strategy for directing stem cell differentiation?", BIOMEDICINE AND PHARMACOTHERAPY, ELSEVIER, PARIS, FR, vol. 59, no. 3, 1 April 2005 (2005-04-01), pages 132 - 134, XP004861305, ISSN: 0753-3322
Attorney, Agent or Firm:
ALTMAN, Daniel, E. et al. (MARTENS OLSON & BEAR, LL,2040 Main Street, Fourteenth Floor, Irvine CA, US)
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