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Title:
GENERATION AND USE OF CANDIDA AND CARBONYL REDUCTASE THEREOF
Document Type and Number:
WIPO Patent Application WO/2016/138641
Kind Code:
A1
Abstract:
A candida parapsilosis CGMCC 9630. The bacterial strain expresses a carbonyl reductase and a coding gene and amino acid sequence of the carbonyl reductase, comprises a recombinant expression vector and a recombinant expression conversion medium of a gene sequence, and utilizes a whole cell of the candida parapsilosis, the carbonyl reductase thereof or the corresponding recombinant conversion medium to serve as a catalyst to catalyze an asymmetric reduction of a prochiral carbonyl compound, especially for reduction of 6-carbonyl-8-halogenated octanoate to prepare (R)-α-thioctic acid and to synthesize a precursor (R)-6-carbonyl-8-halogenated octanoate. Compared to other methods for preparing the (R)-6-carbonyl-8-halogenated octanoate via asymmetric reduction, the method has a high substrate concentration, a mild reaction condition, a high productivity and a high product optical purity, is environmentally friendly, and has a good application prospect in industrial production of (R)-α-thioctic acid.

Inventors:
XU JIANHE (CN)
ZHANG YUJUN (CN)
ZHENG GAOWEI (CN)
PAN JIANG (CN)
Application Number:
PCT/CN2015/073615
Publication Date:
September 09, 2016
Filing Date:
March 04, 2015
Export Citation:
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Assignee:
UNIV EAST CHINA SCIENCE & TECH (CN)
International Classes:
C12N9/02; C12N1/16; C12N15/53; C12P7/42; C12R1/72
Foreign References:
CN1884502A2006-12-27
CN103849574A2014-06-11
Other References:
DATABASE NCBI 26 October 2011 (2011-10-26), GUIDA, A. ET AL.: "hypothetical protein CPAR2_104460 [Candida parapsilosis].", Database accession no. CCE40410.1
DATABASE NCBI 26 October 2011 (2011-10-26), GUIDA, A. ET AL.: "Candida parapsilosis strain CDC317 annotated contig 005569.", Database accession no. HE605203.1.
Attorney, Agent or Firm:
SHANGHAI PATENT & TRADEMARK LAW OFFICE, LLC (CN)
上海专利商标事务所有限公司 (CN)
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