Title:
GENES ENCODING CAROTENOID COMPOUNDS
Document Type and Number:
WIPO Patent Application WO2003016503
Kind Code:
A3
Abstract:
Genes have been isolated from Pantoea stewartii encoding geranylgeranyl pyrophosphate (GGPP) synthase (crtE), phytoene synthase (crtB), phytoene desaturase (crtl), lycopene cyclase (crtY), beta -carotene hydroxylase(crtZ), and zeaxanthin glucosyl transferase(crtX) activity. The genes and their products are useful for the conversion of phytoene to the carotenoids. Vectors containing those DNA segments, host cells containing the vectors and methods for producing those enzymes and beta -carotene by recombinant DNA technology in transformed host organisms are disclosed.
Inventors:
BRZOSTOWICZ PATRICIA C (US)
CHENG QIONG (US)
PICATAGGIO STEPHEN K (US)
ROUVIERE PIERRE E (US)
CHENG QIONG (US)
PICATAGGIO STEPHEN K (US)
ROUVIERE PIERRE E (US)
Application Number:
PCT/US2002/026647
Publication Date:
September 30, 2004
Filing Date:
August 15, 2002
Export Citation:
Assignee:
DU PONT (US)
BRZOSTOWICZ PATRICIA C (US)
CHENG QIONG (US)
PICATAGGIO STEPHEN K (US)
ROUVIERE PIERRE E (US)
BRZOSTOWICZ PATRICIA C (US)
CHENG QIONG (US)
PICATAGGIO STEPHEN K (US)
ROUVIERE PIERRE E (US)
International Classes:
C12N15/52; (IPC1-7): C12N1/12; C07H21/04; C12N1/14; C12N1/16; C12N1/18; C12N5/10; C12N9/88; C12N15/00; C12Q1/68
Domestic Patent References:
| WO2002018617A2 | 2002-03-07 | |||
| WO2002079395A2 | 2002-10-10 |
Foreign References:
| US5429939A | 1995-07-04 |
Other References:
MISAWA ET AL: "Elucidation of the erwinia uredovora carenoid biosynthetic pathway by functional analysis of gene products expressed in Escherichia coli", J. BACTERIOL., vol. 172, no. 12, December 1990 (1990-12-01), pages 6704 - 6712, XP002067963
LIN ET AL: "Transcriptional activation of flanking sequences by Tn1000 insertion", MOL GEN GENET., vol. 245, no. 4, 15 November 1994 (1994-11-15), pages 417 - 423, XP002978767
BARTLEY ET AL: "Two Arabidopsis thaliana carotene desaturases, phytoene desaturase and dzeta-carotene desaturase, expressed in Escherichia coli, catalyze a poly-cis pathway to yield pro-lycopene", EUR. J. BIOCHEM., vol. 259, 1999, pages 396 - 403, XP000925505
MISAWA ET AL: "Production of beta-carotene in zymomonas mobilis and Agrobacterium tumefaciens by introduction of the biosynthesis gene from Erwina uredovora", APPL. ENVIRON. MICROBIOL., vol. 57, no. 6, June 1991 (1991-06-01), pages 1847 - 1849, XP002979016
ARMSTRONG ET AL: "Genetic and biochemical characterization of carotenoid biosynthesismutants of rhododbacter capsulatus", J. BIOL. CHEM., vol. 265, no. 14, 15 May 1990 (1990-05-15), pages 8329 - 8338, XP000881086
LIN ET AL: "Transcriptional activation of flanking sequences by Tn1000 insertion", MOL GEN GENET., vol. 245, no. 4, 15 November 1994 (1994-11-15), pages 417 - 423, XP002978767
BARTLEY ET AL: "Two Arabidopsis thaliana carotene desaturases, phytoene desaturase and dzeta-carotene desaturase, expressed in Escherichia coli, catalyze a poly-cis pathway to yield pro-lycopene", EUR. J. BIOCHEM., vol. 259, 1999, pages 396 - 403, XP000925505
MISAWA ET AL: "Production of beta-carotene in zymomonas mobilis and Agrobacterium tumefaciens by introduction of the biosynthesis gene from Erwina uredovora", APPL. ENVIRON. MICROBIOL., vol. 57, no. 6, June 1991 (1991-06-01), pages 1847 - 1849, XP002979016
ARMSTRONG ET AL: "Genetic and biochemical characterization of carotenoid biosynthesismutants of rhododbacter capsulatus", J. BIOL. CHEM., vol. 265, no. 14, 15 May 1990 (1990-05-15), pages 8329 - 8338, XP000881086
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