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Title:
GENETIC ANALYSIS METHOD
Document Type and Number:
WIPO Patent Application WO/2017/169240
Kind Code:
A1
Abstract:
Provided is a highly reliable genetic analysis method using flow cytometry. This genetic analysis method comprises: a staining step in which a cell is stained; a sorting step in which cell-derived first information of a sample solution is acquired by means of flow cytometry, said first information is analyzed in accordance with a determined extraction condition, and on the basis of the analysis result, a target cell is sorted into a container in which a plurality of wells are arrayed; an amplification step in which the DNA of the cell sorted into the container is amplified; an analysis step in which genetic analysis is performed on the amplified DNA; and a condition determination step in which on the basis of second information acquired in the amplification step and/or third information acquired in the analysis step, the extraction condition is redetermined.

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Inventors:
KANEKO YASUHISA (JP)
Application Number:
PCT/JP2017/005634
Publication Date:
October 05, 2017
Filing Date:
February 16, 2017
Export Citation:
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Assignee:
FUJIFILM CORP (JP)
International Classes:
C12Q1/68
Foreign References:
JP2005315862A2005-11-10
JPH09508270A1997-08-26
JP2009207392A2009-09-17
Other References:
ZIEGLER, BENEDIKT L. ET AL.: "Surface Antigen Expression on CD 34+ Cord Blood Cells: Comparative Analysis by Flow Cytometry and Limiting Dilution (LD) RT-PCR of Chymopapain- Treated or untreated Cells", CYTOMETRY, vol. 25, 1996, pages 46 - 57
HARVEY, BARRETT R. ET AL.: "Anchored periplasmic expression, a versatile technology for the isolation of high-affinity antibodies from Escherichia coli-expressed libraries", PNAS, vol. 101, no. 25, 22 June 2004 (2004-06-22), pages 9193 - 9198, XP002358368
Attorney, Agent or Firm:
NAKASHIMA Junko et al. (JP)
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