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Title:
GENETICALLY ENGINEERED BACTERIUM AND PRODUCTION METHOD FOR PRODUCING LACTYL-N-NEOTETRAOSE
Document Type and Number:
WIPO Patent Application WO/2022/228169
Kind Code:
A1
Abstract:
A genetically engineered bacterium and a production method for producing lactyl-N-neotetraose, relating to the technical field of metabolic engineering and food biology. In order to solve the existing problem of low yield of producing lactyl-N-neotetraose by using a microbial method, by means of exogenous expression of lgtA and lgtB, overexpression of lacY, pgm, gale, galT, and galK in a lactyl-N-neotetraose synthesis pathway is reasonably combined and regulated and controlled, a lacZ expression in Escherichia coli hosts is knocked out, and a carbon source configuration in the culture process is optimized, thereby achieving the purpose of regulating and controlling the carbon flux of a metabolic pathway and improving the yield of the lactyl-N-neotetraose. In shake flask experiments, the capability for Escherichia coli to produce lactyl-N-neotetraose is increased from 304 mg/L to 1031 mg/L.

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Inventors:
ZHANG TAO (CN)
JIANG BO (CN)
HU MIAOMIAO (CN)
LI MENGLI (CN)
Application Number:
PCT/CN2022/087318
Publication Date:
November 03, 2022
Filing Date:
April 18, 2022
Export Citation:
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Assignee:
UNIV JIANGNAN (CN)
International Classes:
C12N1/21; C12P19/26
Foreign References:
CN113136357A2021-07-20
US20020001831A12002-01-03
CN112654697A2021-04-13
CN111548979A2020-08-18
CN109825465A2019-05-31
CN108410787A2018-08-17
US20080145899A12008-06-19
CN109735480A2019-05-10
Other References:
DONG XIAOMIN, LI NAN, LIU ZHENMIN, LV XUEQIN, LI JIANGHUA, DU GUOCHENG, WANG MIAO, LIU LONG: "Modular pathway engineering of key precursor supply pathways for lacto-N-neotetraose production in Bacillus subtilis", BIOTECHNOLOGY FOR BIOFUELS, vol. 12, no. 1, 1 December 2019 (2019-12-01), pages 1 - 11, XP055980699, DOI: 10.1186/s13068-019-1551-3
Attorney, Agent or Firm:
HARBIN SHINEIP INTELLECTUAL PROPERTY AGENCY CO., LTD. (CN)
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