Title:
GENETICALLY ENGINEERED BACTERIUM FOR REALIZING HIGH YIELD OF L-ISOLEUCINE AND METHOD FOR PRODUCING L-ISOLEUCINE BY MEANS OF FERMENTATION METHOD
Document Type and Number:
WIPO Patent Application WO/2022/242637
Kind Code:
A1
Abstract:
Provided is a method for inexpensively producing L-isoleucine at a higher yield by means of a fermentation method, comprising: using a genetic engineering method to obtain a genetically engineered strain, the genetically engineered strain comprising a threonine deaminase gene substantially releasing the inhibition of L-isoleucine and/or an acetylated hydroxy acid synthetase III gene substantially releasing the inhibition of L-isoleucine; and performing fermentation culture on the genetically engineered strain, adding diketobutyric acid or a raw material capable of being converted into diketobutyric acid in a culture process, and separating L-isoleucine from a culture after the end of culturing. Further provided is a genetically engineered strain for realizing high yield of L-isoleucine.
Inventors:
ZHANG KECHUN (CN)
WANG JINYU (CN)
WANG JILONG (CN)
WANG JINYU (CN)
WANG JILONG (CN)
Application Number:
PCT/CN2022/093294
Publication Date:
November 24, 2022
Filing Date:
May 17, 2022
Export Citation:
Assignee:
UNIV WESTLAKE (CN)
International Classes:
C12P13/06; A23K10/18; A23L33/135; A23L33/14; A61K31/198; C12N1/19; C12N1/21; C12R1/07; C12R1/15; C12R1/19; C12R1/465
Foreign References:
| CN114410701A | 2022-04-29 | |||
| CN110305829A | 2019-10-08 | |||
| CN1117524A | 1996-02-28 |
Other References:
LIANGHONG YIN, XIAOQING HU, DAQING XU, JIANFEI NING, JIAN CHEN, XIAOYUAN WANG: "Co-expression of feedback-resistant threonine dehydratase and acetohydroxy acid synthase increase l-isoleucine production in Corynebacterium glutamicum", METABOLIC ENGINEERING, ACADEMIC PRESS, AMSTERDAM, NL, vol. 14, no. 5, 1 September 2012 (2012-09-01), AMSTERDAM, NL, pages 542 - 550, XP055282708, ISSN: 1096-7176, DOI: 10.1016/j.ymben.2012.06.002
陈林 (CHEN LIN): "大肠杆菌苏氨酸脱氨酶变构调控的理性设计与改造 (Rational Design and Reengineering of Allosteric Regulation of Threonine Deaminase from Escherichia Coli)", 中国优秀博硕士学位论文全文数据库(硕士) 工程科技I辑 (CHINESE SELECTED DOCTORAL DISSERTATIONS AND MASTER’S THESES FULL-TEXT DATABASES (DOCTORAL), ENGINEERING SCIENCE & TECHNOLOGY I), no. 05, 15 May 2013 (2013-05-15)
PARK JIN HWAN, OH JAE EUN, LEE KWANG HO, KIM JI YOUNG, LEE SANG YUP: "Rational Design of Escherichia coli for L-Isoleucine Production", ACS SYNTHETIC BIOLOGY, AMERICAN CHEMICAL SOCIETY, WASHINGTON DC ,USA, vol. 1, no. 11, 16 November 2012 (2012-11-16), Washington DC ,USA , pages 532 - 540, XP055918273, ISSN: 2161-5063, DOI: 10.1021/sb300071a
陈林 (CHEN LIN): "大肠杆菌苏氨酸脱氨酶变构调控的理性设计与改造 (Rational Design and Reengineering of Allosteric Regulation of Threonine Deaminase from Escherichia Coli)", 中国优秀博硕士学位论文全文数据库(硕士) 工程科技I辑 (CHINESE SELECTED DOCTORAL DISSERTATIONS AND MASTER’S THESES FULL-TEXT DATABASES (DOCTORAL), ENGINEERING SCIENCE & TECHNOLOGY I), no. 05, 15 May 2013 (2013-05-15)
PARK JIN HWAN, OH JAE EUN, LEE KWANG HO, KIM JI YOUNG, LEE SANG YUP: "Rational Design of Escherichia coli for L-Isoleucine Production", ACS SYNTHETIC BIOLOGY, AMERICAN CHEMICAL SOCIETY, WASHINGTON DC ,USA, vol. 1, no. 11, 16 November 2012 (2012-11-16), Washington DC ,USA , pages 532 - 540, XP055918273, ISSN: 2161-5063, DOI: 10.1021/sb300071a
Attorney, Agent or Firm:
CHANGSHA DEQUAN INTELLECTUAL PROPERTY AGENCY OFFICE (GENERAL PARTNERSHIP ) (CN)
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