Title:
GENETICALLY ENGINEERED CELL AND APPLICATION THEREOF
Document Type and Number:
WIPO Patent Application WO/2019/219029
Kind Code:
A1
Abstract:
Disclosed in the present invention is a genetically engineered cell, expressing an exogenous receptor that specifically binds to a target antigen and exogenous CCL21, and capable of further expressing an IL-7R binding protein or exogenous IL-7 that promotes cell proliferation. Also disclosed are an expression construct comprising an exogenous CCL21 expression cassette, and a vector containing same, a virus, and a pharmaceutical composition comprising said cell. Also disclosed is an application for the cell, the expression construct, the vector, and the virus in the preparation of drugs for inhibiting tumours or inhibiting pathogens.
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Inventors:
LI ZONGHAI (CN)
LUO HONG (CN)
JIANG HUA (CN)
WANG HUAMAO (CN)
LUO HONG (CN)
JIANG HUA (CN)
WANG HUAMAO (CN)
Application Number:
PCT/CN2019/087077
Publication Date:
November 21, 2019
Filing Date:
May 15, 2019
Export Citation:
Assignee:
CARSGEN THERAPEUTICS CO LTD (CN)
SHANGHAI CANCER INST (CN)
SHANGHAI CANCER INST (CN)
International Classes:
C12N5/10; A61K35/17; A61P35/00
Domestic Patent References:
| WO2017186121A1 | 2017-11-02 | |||
| WO2018006882A1 | 2018-01-11 | |||
| WO2015172339A1 | 2015-11-19 | |||
| WO2018018958A1 | 2018-02-01 | |||
| WO2017159736A1 | 2017-09-21 |
Foreign References:
| CN107635569A | 2018-01-26 | |||
| CN102656263A | 2012-09-05 | |||
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Other References:
COMERFORD I. ET AL.: "A Myriad of Functions and Complex Regulation of The CCR7/CCL19/ CCL21 Chemokine Axis in Adaptive Immune System", CYTOKINE & GROWTH FACTOR REVIEWS, vol. 24, no. 3, 30 June 2013 (2013-06-30), pages 269 - 283, XP028675599, DOI: 10.1016/j.cytogfr.2013.03.001
NATURE BIOTECHNOLOGY, vol. 36, no. 4, 2018, pages 346 - 351
FREDERICKM.AUSUBEL: "Current Protocols in Molecular Biology", 2000, WILEYAND SONINC, LIBRARY OF CONGRESS
SAMBROOK ET AL.: "Molecular Cloning: A Laboratory Manual", 2001, COLD SPRING HARBOR LABORATORY PRESS
M.J.GAITED.: "A Practical Guide To Molecular Cloning", 1984
J. H. MILLERM. P. CALOSEDS.: "Immunochemical Methods In Cell And Molecular Biology", 1987, COLD SPRING HARBOR LABORATORY
"Hand book Of Experimental Immunology", vol. I-IV, 1986, COLD SPRING HARBOR LABORATORY PRESS
"Methods In ENZYMOLOGY", vol. 185, ACADEMIC PRESS, INC., article "Gene Expression Technology"
"GenBank", Database accession no. AAA62478.2
DAVIES JK. ET AL.: "Combining CD 19 redirection and alloanergization to generate tumor-specific human T cells for allogeneic cell therapy of B-cell malignancies", CANCER RES, vol. 70, no. 10, 2010, pages OF1 - 10, XP055250493, DOI: 10.1158/0008-5472.CAN-09-3845
J. SAMBROOK ET AL.: "Molecular Cloning Experiment Guide", 2002, SCIENCE PRESS
COMERFORD I. ET AL.: "A Myriad of Functions and Complex Regulation of The CCR7/CCL19/ CCL21 Chemokine Axis in Adaptive Immune System", CYTOKINE & GROWTH FACTOR REVIEWS, vol. 24, no. 3, 30 June 2013 (2013-06-30), pages 269 - 283, XP028675599, DOI: 10.1016/j.cytogfr.2013.03.001
SHAHANI NOOR;EMMA H WILSON: "Role of C-C chemokine receptor type 7 and its ligands during neuroinflammation", JOURNAL OF NEUROINFLAMMATION, vol. 9, no. 1, 25 April 2012 (2012-04-25), GB , pages 77, XP021133281, ISSN: 1742-2094, DOI: 10.1186/1742-2094-9-77
STEFANIE SIEGERT, ET. AL.: "Positive and negative regulation of T cell responses by fibroblastic reticular cells within paracortical regions of lymph nodes", FRONTIERS IN IMMUNOLOGY, VOL. 3, 11 September 2012 (2012-09-11), pages 285, XP055428532, Retrieved from the Internet [retrieved on 20171124], DOI: 10.3389/fimmu.2012.00285
LUO HONG, SU JINGWEN, SUN RUIXIN, SUN YANSHA, WANG YI, DONG YIWEI, SHI BIZHI, JIANG HUA, LI ZONGHAI: "Coexpression of IL7 and CCL21 Increases Efficacy of CAR-T Cells in Solid Tumors without Requiring Preconditioned Lymphodepletion", CLINICAL CANCER RESEARCH, vol. 26, no. 20, 15 October 2020 (2020-10-15), US, pages 5494 - 5505, XP055921328, ISSN: 1078-0432, DOI: 10.1158/1078-0432.CCR-20-0777
VICARI A P,ET AL: "Antitumor effects of the mouse chemokine 6Ckine/SLC through angiostatic and immunological mechanisms.", THE JOURNAL OF IMMUNOLOGY, vol. 165, no. 4, 15 August 2000 (2000-08-15), US , pages 1992 - 2000, XP002248080, ISSN: 0022-1767
See also references of EP 3822345A4
NATURE BIOTECHNOLOGY, vol. 36, no. 4, 2018, pages 346 - 351
FREDERICKM.AUSUBEL: "Current Protocols in Molecular Biology", 2000, WILEYAND SONINC, LIBRARY OF CONGRESS
SAMBROOK ET AL.: "Molecular Cloning: A Laboratory Manual", 2001, COLD SPRING HARBOR LABORATORY PRESS
M.J.GAITED.: "A Practical Guide To Molecular Cloning", 1984
J. H. MILLERM. P. CALOSEDS.: "Immunochemical Methods In Cell And Molecular Biology", 1987, COLD SPRING HARBOR LABORATORY
"Hand book Of Experimental Immunology", vol. I-IV, 1986, COLD SPRING HARBOR LABORATORY PRESS
"Methods In ENZYMOLOGY", vol. 185, ACADEMIC PRESS, INC., article "Gene Expression Technology"
"GenBank", Database accession no. AAA62478.2
DAVIES JK. ET AL.: "Combining CD 19 redirection and alloanergization to generate tumor-specific human T cells for allogeneic cell therapy of B-cell malignancies", CANCER RES, vol. 70, no. 10, 2010, pages OF1 - 10, XP055250493, DOI: 10.1158/0008-5472.CAN-09-3845
J. SAMBROOK ET AL.: "Molecular Cloning Experiment Guide", 2002, SCIENCE PRESS
COMERFORD I. ET AL.: "A Myriad of Functions and Complex Regulation of The CCR7/CCL19/ CCL21 Chemokine Axis in Adaptive Immune System", CYTOKINE & GROWTH FACTOR REVIEWS, vol. 24, no. 3, 30 June 2013 (2013-06-30), pages 269 - 283, XP028675599, DOI: 10.1016/j.cytogfr.2013.03.001
SHAHANI NOOR;EMMA H WILSON: "Role of C-C chemokine receptor type 7 and its ligands during neuroinflammation", JOURNAL OF NEUROINFLAMMATION, vol. 9, no. 1, 25 April 2012 (2012-04-25), GB , pages 77, XP021133281, ISSN: 1742-2094, DOI: 10.1186/1742-2094-9-77
STEFANIE SIEGERT, ET. AL.: "Positive and negative regulation of T cell responses by fibroblastic reticular cells within paracortical regions of lymph nodes", FRONTIERS IN IMMUNOLOGY, VOL. 3, 11 September 2012 (2012-09-11), pages 285, XP055428532, Retrieved from the Internet
LUO HONG, SU JINGWEN, SUN RUIXIN, SUN YANSHA, WANG YI, DONG YIWEI, SHI BIZHI, JIANG HUA, LI ZONGHAI: "Coexpression of IL7 and CCL21 Increases Efficacy of CAR-T Cells in Solid Tumors without Requiring Preconditioned Lymphodepletion", CLINICAL CANCER RESEARCH, vol. 26, no. 20, 15 October 2020 (2020-10-15), US, pages 5494 - 5505, XP055921328, ISSN: 1078-0432, DOI: 10.1158/1078-0432.CCR-20-0777
VICARI A P,ET AL: "Antitumor effects of the mouse chemokine 6Ckine/SLC through angiostatic and immunological mechanisms.", THE JOURNAL OF IMMUNOLOGY, vol. 165, no. 4, 15 August 2000 (2000-08-15), US , pages 1992 - 2000, XP002248080, ISSN: 0022-1767
See also references of EP 3822345A4
Attorney, Agent or Firm:
SHANGHAI BESHINING LAW OFFICE (CN)
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