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Title:
GENETICALLY ENGINEERED STRAIN OF HIGH YIELD PHENAZINE-1-CARBOXAMIDE, CONSTRUCTION METHOD FOR SAME, AND USES THEREOF
Document Type and Number:
WIPO Patent Application WO/2018/036479
Kind Code:
A1
Abstract:
A genetically engineered strain of phenazine-1-carboxamide (PCN), a preparation method for same, and uses thereof. The genetically engineered strain specifically is a genetically engineered strain produced by knocking off either or both of lon gene and parS gene of the genome of Pseudomonas chlororaphis HT66 CCTCC NO: M 2013467 and of a derivative thereof; or a genetically engineered strain produced by knocking off either or both of lon gene and parS gene of the genome of rpeA and psrA single-mutation strain or double-mutation strain of Pseudomonas chlororaphis HT66 CCTCC NO: M 2013467; or one by knocking off pykA gene of Pseudomonas chlororaphis HT66 CCTCC NO: M 2013467 and of a derivative thereof, where the single-mutation strain is HT66ΔrpeA or HT66ΔpsrA, and the double-mutation strain is T66ΔrpeAΔpsrA. When the lon and parS genes in the genome of the Pseudomonas chlororaphis are deleted, the fermentation yield of phenazine-1-carboxamide can reach up to 3594 mg/L, thus being applicable in industrial production and agricultural application of phenazine-1-carboxamide.

Inventors:
PENG HUASONG (CN)
ZHANG XUEHONG (CN)
Application Number:
PCT/CN2017/098480
Publication Date:
March 01, 2018
Filing Date:
August 22, 2017
Export Citation:
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Assignee:
UNIV SHANGHAI JIAOTONG (CN)
International Classes:
C12N1/21; C12N15/78; C12P17/12; C12R1/38
Foreign References:
CN106635937A2017-05-10
CN107043730A2017-08-15
CN103642714A2014-03-19
CN104017744A2014-09-03
EP1155617A12001-11-21
Other References:
LIU, K. ET AL.: "Genetic Engineering of Pseudomonas Chlororaphis GP72 for the Enhanced Production of 2-Hydroxyphenazine", MICROBIAL CELL FACTORIES, vol. 15, 28 July 2016 (2016-07-28), XP055465721
WANG, D. ET AL.: "Transcriptome Profiling Reveals Links Between ParS/ParR, Me-xEF-OprN, and Quorum Sensing in the Regulation of Adaptation and Virulence in Pseudomonas Aeruginosa", BMC GENOMICS, vol. 14, 13 September 2013 (2013-09-13), XP021158558
GIRARD, G. ET AL.: "Regulatory Roles of psrA and rpoS in Phenazine-l-carboxamide Synthesis by Pseudomonas Chlororaphis PCL1391", MICROBIOLOGY, vol. 152, 31 December 2006 (2006-12-31), XP055465723
CHEN, Y. ET AL.: "Comparative Genomic Analysis and Phenazine Production of Pseudomonas Chlororaphis, a Plant Growth-Promoting Rhizobacterium", GENOMICS DATA, vol. 4, 22 January 2015 (2015-01-22), XP055465724
WANG, D. ET AL.: "Differential Regulation of Phenazine Biosynthesis by RpeA and RpeB in Pseudomonas Chlororaphis 30-84", MICROBIOLOGY, vol. 158, 31 December 2012 (2012-12-31), pages 1745 - 1757, XP055465727
ZHANG, PINGYUAN ET AL.: "Mutagenesis and Genetic Engineering Breed of Pseudomonas Chlororaphis with High Yield of Phenaizne-1-Carboximade", JOURNAL OF SHANGHAI JIAOTONG UNIVERSITY (AGRICULTURAL SCIENCE, vol. 33, no. 2, 30 April 2015 (2015-04-30)
OUYANG, YI ET AL.: "Function of the PhzI-PhzR System in Pseudomonas Chlororaphis HT66", J OURNAL OF SHANGHAI JIAOTONG UNIVERSITY (AGRICULTURAL SCIENCE, vol. 34, no. 1, 29 February 2016 (2016-02-29), pages 5 - 10
CHEN, YAWEN ET AL.: "the Effects of luxR_19 on the Synthesis of Phenzaine-1-Carboxamide in Pseudomonas Chlororaphis HT66", BIOTECHNOLOGY BULLETIN, vol. 31, no. 8, 31 December 2015 (2015-12-31), pages 166 - 173, XP055465736
Attorney, Agent or Firm:
SHANGHAI HANGSOME INTELLECTUAL PROPERTY LTD. (CN)
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