Title:
GENETICALLY ENGINEERED STRAIN FOR PRODUCING POLYLACTIC ACID AND METHOD FOR PRODUCING POLYLACTIC ACID
Document Type and Number:
WIPO Patent Application WO/2023/051077
Kind Code:
A1
Abstract:
Provided are a genetically engineered strain for producing polylactic acid and a method for producing polylactic acid. The method comprises: synthesizing a D-lactate dehydrogenase gene, a propionyl-CoA transferase gene and a polyhydroxyalkanoate synthase gene into a target gene, and performing double enzymatic cleavage on same and a vector, purifying, and transforming a competent cell to obtain a recombinant plasmid; extracting DNA of the recombinant plasmid and introducing the DNA into an engineering strain, and extracting and verifying to obtain an engineering cell strain; and then culturing same in a culture medium, introducing carbon dioxide and light into the culture medium, centrifuging, drying, and recycling. According to the present invention, by modifying natural cyanobacteria and using a high density fermentation strategy, the biomass of the cyanobacteria is greatly improved. According to the present invention, the problem in the prior art that only a sugar-derived raw material can be used to produce polylactic acid is solved.
Inventors:
TAO FEI (CN)
TAN CHUNLIN (CN)
XU PING (CN)
TAN CHUNLIN (CN)
XU PING (CN)
Application Number:
PCT/CN2022/113485
Publication Date:
April 06, 2023
Filing Date:
August 19, 2022
Export Citation:
Assignee:
UNIV SHANGHAI JIAOTONG (CN)
International Classes:
C12N15/74; C12N1/21; C12N15/52; C12N15/53; C12N15/54; C12P7/62; C12R1/01
Foreign References:
| CN113736814A | 2021-12-03 | |||
| CN114703067A | 2022-07-05 | |||
| CN107267476A | 2017-10-20 | |||
| US20120220007A1 | 2012-08-30 | |||
| US20150315599A1 | 2015-11-05 | |||
| US20090203070A1 | 2009-08-13 | |||
| CN202220201535U | 2022-01-25 |
Other References:
XIAO, JIANXUN ET AL.: "Development and Prospect of Cyanobacteria Carbon Fixation and Synthesis of Lactic Acid Technology", CHINESE JOURNAL OF BIOTECHNOLOGY, vol. 37, no. 4, 25 April 2021 (2021-04-25), XP009545013, ISSN: 1000-3061
JUNG ET AL., BIOTECHNOLOGY & BIOENGINEERING, vol. 105, no. 1, 2010, pages 161 - 171
LI, C.TAO, F.NI, J. ET AL.: "Enhancing the light-driven production of D-lactate by engineering cyanobacterium using a combinational strategy", SCI REP, vol. 5, 2015, pages 9777
JUNG ET AL., BIOTECHNOLOGY & BIOENGINEERING, vol. 105, no. 1, 2010, pages 161 - 171
LI, C.TAO, F.NI, J. ET AL.: "Enhancing the light-driven production of D-lactate by engineering cyanobacterium using a combinational strategy", SCI REP, vol. 5, 2015, pages 9777
Attorney, Agent or Firm:
SUNRAY INTELLECTUAL PROPERTY ATTORNEYS (CN)
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