GENOME ASSEMBLY METHOD FOR CONSTRUCTING ULTRALONG CONTINUOUS DNA SEQUENCE

WIPO Patent Application WO/2019/233427

A1

A genome assembly method for constructing an ultralong continuous DNA sequence, comprising: S1, finding an overlapping region between each pair of known DNA sequences; S2, starting from a free end of any anchor sequence fragment, extending the anchor sequence fragment with a read sequence overlapping therewith, repeating the extension a plurality of times until a read sequence that can be aligned to an end of another different anchor sequence fragment is found, so as to obtain one or a plurality of pathway sequences; S3, selecting at most one of all the pathway sequences as an effective joining sequence to join the end of the initial anchor sequence fragment to the end of the other ending anchor sequence fragment; S4, using the effective joining sequence to join the initial and corresponding ending anchor sequence fragment; using the joined anchor sequence fragment as a new anchor sequence fragment, or recording a free end of a remaining anchor sequence fragment, and proceeding to S2; and repeating steps S2-S4 to finally form an ultralong continuous DNA sequence.

LIANG CHENGZHI (CN)
DU HUILONG (CN)

PCT/CN2019/090053

December 12, 2019

June 05, 2019

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INST OF GENETICS CHINESE ACADEMY OF SCIENCES (CN)

C12Q1/68

WO2010075570A2

2010-07-01

CN108753765A	2018-11-06
CN104239750A	2014-12-24
CN104239750A	2014-12-24
CN105303068A	2016-02-03
CN105986008A	2016-10-05
US20120295260A1	2012-11-22

CN-KNOWHOW INTELLECTUAL PROPERTY AGENT LIMITED (CN)

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