GENOME EDITING METHOD AND COMPOSITION FOR GENOME EDITING

WIPO Patent Application WO/2024/080067

A1

The present invention provides a novel genome editing means that makes it possible to recognize a novel PAM sequence in a eukaryotic cell genome and site-specifically modify the genome. Provided is a method for site-specifically modifying a target DNA sequence in a eukaryotic cell genome, said method comprising modifying the eukaryotic cell genome in the target DNA sequence by introducing, into the eukaryotic cell, (1) a Cas protein that recognizes a protospacer adjacent motif (PAM) sequence including the base sequence 5'-NNACNN-3' of SEQ ID NO: 1 (where each N represents one arbitrary base independently selected from adenine, cytosine, thymine, and guanine) or a nucleic acid that codes for said Cas protein, and (2) guide RNA that can hybridize to the target DNA sequence in the genome and that can form a complex with the Cas protein and direct sequence specific binding to the target DNA sequence of the complex, or a nucleic acid that codes for the guide RNA.

TERAKAWA TERUHIKO (JP)
YANO TSUBASA (JP)
MITSUDA NOBUTAKA (JP)
NAKAMURA AKIYOSHI (JP)
SUGANO SHIGEO (JP)
ITO SEIICHIRO (JP)
MAKINO YOICHI (JP)

PCT/JP2023/033481

April 18, 2024

September 14, 2023

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INPLANTA INNOVATIONS INC (JP)
AIST (JP)
TOPPAN HOLDINGS INC (JP)

C12N15/09

GASIUNAS GIEDRIUS, YOUNG JOSHUA K., KARVELIS TAUTVYDAS, KAZLAUSKAS DARIUS, URBAITIS TOMAS, JASNAUSKAITE MONIKA, GRUSYTE MANTVYDA M: "A catalogue of biochemically diverse CRISPR-Cas9 orthologs", NATURE COMMUNICATIONS, vol. 11, no. 1, 1 December 2020 (2020-12-01), pages 1 - 10, XP055929454, DOI: 10.1038/s41467-020-19344-1

AOKI, Atsushi et al. (JP)

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