Title:
β-GLUCAN-PRODUCING FUNGUS WITHOUT MELANIN PIGMENT FORMATION ABILITY OR WITH LOW MELANIN PIGMENT FORMATION ABILITY, METHOD FOR ARTIFICIALLY PRODUCING SAID FUNGUS, β-GLUCAN PRODUCED UTILIZING SAID FUNGUS, AND METHOD FOR PRODUCING SAID Β-GLUCAN
Document Type and Number:
WIPO Patent Application WO/2017/221905
Kind Code:
A1
Abstract:
[Problem] To provide a β-glucan-producing fungus without a melanin pigment formation ability or with a low melanin pigment formation ability, which is mutated from a common β-glucan-producing fungus, does not have a melanin pigment formation ability or has a lower melanin pigment formation ability compared with an initial state before the mutation treatment of the microorganism, and has a β-glucan production ability. [Solution] The method comprises: a gene mutation treatment step of subjecting a fungus Aureobasidium pullulans (a black yeast fungus) to a mutation treatment by means of a physical or chemical treatment for causing a mutation in a gene of the fungus; and a mutant strain selection step of screening for a strain in which the occurrence of melanin formation is not observed, in both of the culturing of a mutant strain produced in the gene mutation treatment step in the presence of a tyrosinase pathway inhibitor and the culturing of the mutant strain in the presence of a polyketide pathway inhibitor. This double-screening enables the inhibition of the formation of a melanin pigment and, consequently, enables the production of a β-glucan-producing strain which has a β-glucan production ability and which does not have a melanin pigment formation ability or has a low melanin pigment formation ability. For example, a radioactive irradiation treatment with gamma ray is employed as the mutation treatment.
Inventors:
SUZUKI TOSHIO (JP)
KITAMURA KENJI (JP)
KITAMURA KENJI (JP)
Application Number:
PCT/JP2017/022596
Publication Date:
December 28, 2017
Filing Date:
June 19, 2017
Export Citation:
Assignee:
SUZUKI TOSHIO (JP)
International Classes:
C12N1/16; C12P19/04
Foreign References:
| JP2006075076A | 2006-03-23 | |||
| JPH0956391A | 1997-03-04 | |||
| JP2004329077A | 2004-11-25 |
Other References:
KAORI MIYAWAKI: "Melanin Hi Sanseisei Aureobasidium-zoku Kobo no Bunri to Sono Tokusei", PROCEEDINGS OF THE ANNUAL MEETING OF JAPAN SOCIETY FOR BIOSCIENCE, BIOTECHNOLOGY , AND AGROCHEMISTRY, vol. 2007, 2007, pages 288
Attorney, Agent or Firm:
NAGAI,Michiaki (JP)
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