Title:
GLUCOSE DEHYDROGENASE
Document Type and Number:
WIPO Patent Application WO/2018/062103
Kind Code:
A1
Abstract:
The present invention addresses the problem of providing novel FAD-GDH having high practicality. Provided is glucose dehydrogenase having the amino acid sequence of (a) or (b) as follows. (a) The amino acid sequence of SEQ ID NO. 1 in which the lysine at position 354 has been substituted with a valine, isoleucine, proline, or glutamine, or (b) an amino acid sequence which is at least 80% identical to the amino acid sequence of (a), wherein a polypeptide having said amino acid sequence has improved low-temperature reactivity in terms of glucose dehydrogenase activity compared to a polypeptide having the amino acid sequence of SEQ ID NO. 1.
Inventors:
NISHIO KYOICHI (JP)
KOJIMA YUZO (JP)
YAMAGUCHI SHOTARO (JP)
KOJIMA YUZO (JP)
YAMAGUCHI SHOTARO (JP)
Application Number:
PCT/JP2017/034547
Publication Date:
April 05, 2018
Filing Date:
September 25, 2017
Export Citation:
Assignee:
AMANO ENZYME INC (JP)
International Classes:
C12N9/04; C12N1/15; C12N1/19; C12N1/21; C12N5/10; C12N15/09; C12P21/02; C12Q1/32; G01N33/66
Domestic Patent References:
| WO2015060150A1 | 2015-04-30 | |||
| WO2004058958A1 | 2004-07-15 | |||
| WO2007139013A1 | 2007-12-06 | |||
| WO2017077924A1 | 2017-05-11 |
Foreign References:
| JP2011217731A | 2011-11-04 | |||
| JP2016116488A | 2016-06-30 | |||
| JP2015146773A | 2015-08-20 |
Attorney, Agent or Firm:
HAGINO Mikiharu (JP)
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