Title:
GLUTAMINE SYNTHETASE MUTANT HAVING GLUFOSINATE-AMMONIUM RESISTANCE, NUCLEIC ACID MOLECULE AND USE
Document Type and Number:
WIPO Patent Application WO/2023/040565
Kind Code:
A1
Abstract:
Provided are a glutamine synthetase mutant having glufosinate-ammonium resistance, a nucleic acid molecule and the use, which relate to the technical field of genetic engineering. The glutamine synthetase mutant can be obtained by means of mutation at the nth site of wild glutamine synthetase. After mutation, the nth site is transformed into C, E, F, I, M, N, P, S and Y or is deleted, and the mutation can endow the glutamine synthetase with glufosinate-ammonium resistance suitable for commercial application. The glutamine synthetase mutant has application potential in terms of constructing expression vectors for transforming plants and cultivating glufosinate-ammonium-resistant crops.
Inventors:
DENG LONGQUN (CN)
ZHANG ZHEN (CN)
CHEN RONG (CN)
HOU QINGJIANG (CN)
XU NANFEI (CN)
ZHANG ZHEN (CN)
CHEN RONG (CN)
HOU QINGJIANG (CN)
XU NANFEI (CN)
Application Number:
PCT/CN2022/113148
Publication Date:
March 23, 2023
Filing Date:
August 17, 2022
Export Citation:
Assignee:
GEVOTO LLC (CN)
International Classes:
C12N9/00; C12N1/21; C12N15/52; C12N15/70; C12R1/19
Domestic Patent References:
| WO2019118726A2 | 2019-06-20 |
Foreign References:
| CN113736757A | 2021-12-03 | |||
| CN112574967A | 2021-03-30 | |||
| CN110229794A | 2019-09-13 |
Other References:
REN, YAN ET AL.: "Screening of Rice(Oryza sativa L.)glufosinate-tolerant Mutants and Preliminary Study on Its Mechanism", JOURNAL OF BIOLOGY, vol. 38, no. 2, 30 April 2021 (2021-04-30), pages 51 - 55, XP093048938, DOI: 10.3969/j.issn.2095-1736.2021.02.51
PORNPROM, T ET AL.: "Glutamine synthetasemutation conferring target-site-based resistance to glufosinate in soybean cell selections", PEST MANAG SCI, vol. 65, 18 December 2008 (2008-12-18), pages 216 - 222, XP055947653, DOI: 10.1002/ps.1671
PORNPROM, T ET AL.: "Glutamine synthetasemutation conferring target-site-based resistance to glufosinate in soybean cell selections", PEST MANAG SCI, vol. 65, 18 December 2008 (2008-12-18), pages 216 - 222, XP055947653, DOI: 10.1002/ps.1671
Attorney, Agent or Firm:
CHOFN INTELLECTUAL PROPERTY (CN)
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