Title:
GLYCEROL AND ACETIC ACID CONVERTING CELLS WITH IMPROVED GLYCEROL TRANSPORT
Document Type and Number:
WIPO Patent Application WO/2015/028583
Kind Code:
A3
Abstract:
Cell that is genetically modified comprising: a) one or more nucleotide sequence encoding a glycerol dehydrogenase (E.C. 1.1.1.6); b) one or more nucleotide sequence encoding a dihydroxyacetone kinase (E.C. 2.7.1.28 or E.C. 2.7.1.29) and c) one or more nucleotide sequence encoding a glycerol transporter.
Inventors:
KLAASSEN PAUL (NL)
HARTMAN WOUTER WILLEM ANTONIUS (NL)
HARTMAN WOUTER WILLEM ANTONIUS (NL)
Application Number:
PCT/EP2014/068325
Publication Date:
April 23, 2015
Filing Date:
August 29, 2014
Export Citation:
Assignee:
DSM IP ASSETS BV (NL)
International Classes:
C07K14/445; C07K14/39; C07K14/46; C12N9/04; C12N9/12; C12P7/06; C12P7/10
Domestic Patent References:
| WO2013081456A2 | 2013-06-06 | |||
| WO2012067510A1 | 2012-05-24 | |||
| WO2011149353A1 | 2011-12-01 | |||
| WO2010019882A1 | 2010-02-18 | |||
| WO2008006037A2 | 2008-01-10 | |||
| WO2011124146A1 | 2011-10-13 | |||
| WO2010015122A1 | 2010-02-11 |
Foreign References:
| US20130109070A1 | 2013-05-02 |
Other References:
YU K O ET AL: "Reduction of glycerol production to improve ethanol yield in an engineered Saccharomyces cerevisiae using glycerol as a substrate", JOURNAL OF BIOTECHNOLOGY, ELSEVIER SCIENCE PUBLISHERS, AMSTERDAM, NL, vol. 150, no. 2, 15 October 2010 (2010-10-15), pages 209 - 214, XP027424705, ISSN: 0168-1656, DOI: 10.1016/J.JBIOTEC.2010.09.932
YU K O ET AL: "Engineering of glycerol utilization pathway for ethanol production by Saccharomyces cerevisiae", BIORESOURCE TECHNOLOGY, ELSEVIER BV, GB, vol. 101, no. 11, 1 June 2010 (2010-06-01), pages 4157 - 4161, XP026917458, ISSN: 0960-8524, DOI: 10.1016/J.BIORTECH.2010.01.066
XUE-MING TANG ET AL: "Functional analysis of the Zygosaccharomyces rouxii Fps1p homologue", YEAST, vol. 22, no. 7, 2005, pages 571 - 581, XP055095489, ISSN: 0749-503X, DOI: 10.1002/yea.1232
LIHUA HOU ET AL: "Analysis of Salt-Tolerance Genes in Zygosaccharomyces rouxii", APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, vol. 170, no. 6, 15 May 2013 (2013-05-15), pages 1417 - 1425, XP055095349, ISSN: 0273-2289, DOI: 10.1007/s12010-013-0283-2
SUTHERLAND F C W ET AL: "Characteristics of Fps1-dependent and -independent glycerol transport in Saccharomyces cerevisiae", JOURNAL OF BACTERIOLOGY, AMERICAN SOCIETY FOR MICROBIOLOGY, WASHINGTON, DC; US, vol. 179, no. 24, 1 December 1997 (1997-12-01), pages 7790 - 7795, XP002413260, ISSN: 0021-9193
PRUDENT S ET AL: "The yeast osmosensitive mutant fps1DELTA transformed by the cauliflower BobTIP1;1 aquaporin withstand a hypo-osmotic shock", FEBS LETTERS, ELSEVIER, AMSTERDAM, NL, vol. 579, no. 18, 18 July 2005 (2005-07-18), pages 3872 - 3880, XP027697129, ISSN: 0014-5793
JUN-GUO ZHANG ET AL: "Improvement of acetic acid tolerance and fermentation performance of Saccharomyces cerevisiae by disruption of the FPS1 aquaglyceroporin gene", BIOTECHNOLOGY LETTERS, vol. 33, no. 2, 16 October 2010 (2010-10-16), pages 277 - 284, XP055095158, ISSN: 0141-5492, DOI: 10.1007/s10529-010-0433-3
XU X ET AL: "Elementary Flux Mode Analysis for Optimized Ethanol Yield in Anaerobic Fermentation of Glucose with Saccharomyces cerevisiae", CHINESE JOURNAL OF CHEMICAL ENGINEERING, CHEMICAL INDUSTRY PRESS, BEIJING, CN, vol. 16, no. 1, 1 February 2008 (2008-02-01), pages 135 - 142, XP022856604, ISSN: 1004-9541, DOI: 10.1016/S1004-9541(08)60052-X
A. ZHANG ET AL: "Effect of FPS1 deletion on the fermentation properties of Saccharomyces cerevisiae", LETTERS IN APPLIED MICROBIOLOGY, vol. 44, no. 2, 1 February 2007 (2007-02-01), pages 212 - 217, XP055095159, ISSN: 0266-8254, DOI: 10.1111/j.1472-765X.2006.02041.x
BJORN HOLST ET AL: "GUP1 and its close homologue GUP2, encoding multimembrane-spanning proteins involved in active glycerol uptake in Saccharomyces cerevisiae", MOLECULAR MICROBIOLOGY, vol. 37, no. 1, 1 July 2000 (2000-07-01), pages 108 - 124, XP055023726, ISSN: 0950-382X, DOI: 10.1046/j.1365-2958.2000.01968.x
DATABASE UniProt [online] 26 June 2013 (2013-06-26), "SubName: Full=Stl1p;", XP002718506, retrieved from EBI accession no. UNIPROT:N1PAM1 Database accession no. N1PAM1
DATABASE UniProt [online] 28 July 2009 (2009-07-28), "SubName: Full=ZYRO0E01210p;", XP002718507, retrieved from EBI accession no. UNIPROT:C5E3Y3 Database accession no. C5E3Y3
CÉLIA FERREIRA ET AL: "A Member of the Sugar Transporter Family, Stl1p Is the Glycerol/H Symporter in Saccharomyces cerevisiae", MOLECULAR BIOLOGY OF THE CELL, vol. 16, 9 February 2005 (2005-02-09), pages 2068 - 2076, XP055157384, DOI: 10.1091/mbc.E04
L NEVES ET AL: "Yeast orthologues associated with glycerol transport and metabolism", FEMS YEAST RESEARCH, vol. 5, no. 1, 23 July 2004 (2004-07-23), pages 51 - 62, XP055157387, ISSN: 1567-1356, DOI: 10.1016/j.femsyr.2004.06.012
NEVES L ET AL: "New insights on glycerol transport in Saccharomyces cerevisiae", FEBS LETTERS, ELSEVIER, AMSTERDAM, NL, vol. 565, no. 1-3, 7 May 2004 (2004-05-07), pages 160 - 162, XP004507697, ISSN: 0014-5793, DOI: 10.1016/J.FEBSLET.2004.04.003
CÃ LIA FERREIRA ET AL: "Absence of Gup1p in Saccharomyces cerevisiae results in defective cell wall composition, assembly, stability and morphology", FEMS YEAST RESEARCH, vol. 6, no. 7, 1 November 2006 (2006-11-01), pages 1027 - 1038, XP055000262, ISSN: 1567-1356, DOI: 10.1111/j.1567-1364.2006.00110.x
YU K O ET AL: "Engineering of glycerol utilization pathway for ethanol production by Saccharomyces cerevisiae", BIORESOURCE TECHNOLOGY, ELSEVIER BV, GB, vol. 101, no. 11, 1 June 2010 (2010-06-01), pages 4157 - 4161, XP026917458, ISSN: 0960-8524, DOI: 10.1016/J.BIORTECH.2010.01.066
XUE-MING TANG ET AL: "Functional analysis of the Zygosaccharomyces rouxii Fps1p homologue", YEAST, vol. 22, no. 7, 2005, pages 571 - 581, XP055095489, ISSN: 0749-503X, DOI: 10.1002/yea.1232
LIHUA HOU ET AL: "Analysis of Salt-Tolerance Genes in Zygosaccharomyces rouxii", APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, vol. 170, no. 6, 15 May 2013 (2013-05-15), pages 1417 - 1425, XP055095349, ISSN: 0273-2289, DOI: 10.1007/s12010-013-0283-2
SUTHERLAND F C W ET AL: "Characteristics of Fps1-dependent and -independent glycerol transport in Saccharomyces cerevisiae", JOURNAL OF BACTERIOLOGY, AMERICAN SOCIETY FOR MICROBIOLOGY, WASHINGTON, DC; US, vol. 179, no. 24, 1 December 1997 (1997-12-01), pages 7790 - 7795, XP002413260, ISSN: 0021-9193
PRUDENT S ET AL: "The yeast osmosensitive mutant fps1DELTA transformed by the cauliflower BobTIP1;1 aquaporin withstand a hypo-osmotic shock", FEBS LETTERS, ELSEVIER, AMSTERDAM, NL, vol. 579, no. 18, 18 July 2005 (2005-07-18), pages 3872 - 3880, XP027697129, ISSN: 0014-5793
JUN-GUO ZHANG ET AL: "Improvement of acetic acid tolerance and fermentation performance of Saccharomyces cerevisiae by disruption of the FPS1 aquaglyceroporin gene", BIOTECHNOLOGY LETTERS, vol. 33, no. 2, 16 October 2010 (2010-10-16), pages 277 - 284, XP055095158, ISSN: 0141-5492, DOI: 10.1007/s10529-010-0433-3
XU X ET AL: "Elementary Flux Mode Analysis for Optimized Ethanol Yield in Anaerobic Fermentation of Glucose with Saccharomyces cerevisiae", CHINESE JOURNAL OF CHEMICAL ENGINEERING, CHEMICAL INDUSTRY PRESS, BEIJING, CN, vol. 16, no. 1, 1 February 2008 (2008-02-01), pages 135 - 142, XP022856604, ISSN: 1004-9541, DOI: 10.1016/S1004-9541(08)60052-X
A. ZHANG ET AL: "Effect of FPS1 deletion on the fermentation properties of Saccharomyces cerevisiae", LETTERS IN APPLIED MICROBIOLOGY, vol. 44, no. 2, 1 February 2007 (2007-02-01), pages 212 - 217, XP055095159, ISSN: 0266-8254, DOI: 10.1111/j.1472-765X.2006.02041.x
BJORN HOLST ET AL: "GUP1 and its close homologue GUP2, encoding multimembrane-spanning proteins involved in active glycerol uptake in Saccharomyces cerevisiae", MOLECULAR MICROBIOLOGY, vol. 37, no. 1, 1 July 2000 (2000-07-01), pages 108 - 124, XP055023726, ISSN: 0950-382X, DOI: 10.1046/j.1365-2958.2000.01968.x
DATABASE UniProt [online] 26 June 2013 (2013-06-26), "SubName: Full=Stl1p;", XP002718506, retrieved from EBI accession no. UNIPROT:N1PAM1 Database accession no. N1PAM1
DATABASE UniProt [online] 28 July 2009 (2009-07-28), "SubName: Full=ZYRO0E01210p;", XP002718507, retrieved from EBI accession no. UNIPROT:C5E3Y3 Database accession no. C5E3Y3
CÉLIA FERREIRA ET AL: "A Member of the Sugar Transporter Family, Stl1p Is the Glycerol/H Symporter in Saccharomyces cerevisiae", MOLECULAR BIOLOGY OF THE CELL, vol. 16, 9 February 2005 (2005-02-09), pages 2068 - 2076, XP055157384, DOI: 10.1091/mbc.E04
L NEVES ET AL: "Yeast orthologues associated with glycerol transport and metabolism", FEMS YEAST RESEARCH, vol. 5, no. 1, 23 July 2004 (2004-07-23), pages 51 - 62, XP055157387, ISSN: 1567-1356, DOI: 10.1016/j.femsyr.2004.06.012
NEVES L ET AL: "New insights on glycerol transport in Saccharomyces cerevisiae", FEBS LETTERS, ELSEVIER, AMSTERDAM, NL, vol. 565, no. 1-3, 7 May 2004 (2004-05-07), pages 160 - 162, XP004507697, ISSN: 0014-5793, DOI: 10.1016/J.FEBSLET.2004.04.003
CÃ LIA FERREIRA ET AL: "Absence of Gup1p in Saccharomyces cerevisiae results in defective cell wall composition, assembly, stability and morphology", FEMS YEAST RESEARCH, vol. 6, no. 7, 1 November 2006 (2006-11-01), pages 1027 - 1038, XP055000262, ISSN: 1567-1356, DOI: 10.1111/j.1567-1364.2006.00110.x
Attorney, Agent or Firm:
KLEIBORN, Paul Erik (P.O. Box 4, AA Echt, NL)
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