Title:
GPD-YEAST STRAINS WITH IMPROVED OSMOTOLERANCE
Document Type and Number:
WIPO Patent Application WO/2014/180820
Kind Code:
A3
Abstract:
Yeast cell that is genetically modified comprising: a) a deletion or disruption of one or more nucleotide sequence selected from the group of GPD1, GPD2, GPP1 and GPP2 and b) presence of one or more heterologous nucleotide sequence encoding a NAD+-dependent acetylating acetaldehyde dehydrogenase (E.C. 1.2.1.10.), wherein the yeast cell has an osmotolerance of 0.5 OsM or more.
More Like This:
Inventors:
GUADALUPE MEDINA VICTOR GABRIEL (NL)
METZ BENJAMIN (NL)
OUD BART (NL)
GRAAF VAN DER CHARLOTTE M (NL)
MANS ROBERT (NL)
PRONK JACOBUS THOMAS (NL)
MARIS VAN ANTONIUS JEROEN ADRIAAN (NL)
KLAASSEN PAUL (NL)
METZ BENJAMIN (NL)
OUD BART (NL)
GRAAF VAN DER CHARLOTTE M (NL)
MANS ROBERT (NL)
PRONK JACOBUS THOMAS (NL)
MARIS VAN ANTONIUS JEROEN ADRIAAN (NL)
KLAASSEN PAUL (NL)
Application Number:
PCT/EP2014/059193
Publication Date:
January 08, 2015
Filing Date:
May 06, 2014
Export Citation:
Assignee:
DSM IP ASSETS BV (NL)
International Classes:
C12N1/36; C12N9/02; C12N9/04; C12N9/16; C12N15/01; C12P7/06; C12R1/865
Domestic Patent References:
| WO2011010923A1 | 2011-01-27 |
Other References:
XIANGLIN TAO ET AL: "A Novel Strategy to Construct Yeast Saccharomyces cerevisiae Strains for Very High Gravity Fermentation", PLOS ONE, vol. 7, no. 2, 17 February 2012 (2012-02-17), pages e31235, XP055134829, DOI: 10.1371/journal.pone.0031235
G. HUBMANN ET AL: "Gpd1 and Gpd2 Fine-Tuning for Sustainable Reduction of Glycerol Formation in Saccharomyces cerevisiae", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, vol. 77, no. 17, 1 July 2011 (2011-07-01), pages 5857 - 5867, XP055134657, ISSN: 0099-2240, DOI: 10.1128/AEM.05338-11
V. G. MEDINA ET AL: "Elimination of Glycerol Production in Anaerobic Cultures of a Saccharomyces cerevisiae Strain Engineered To Use Acetic Acid as an Electron Acceptor", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, vol. 76, no. 1, 13 November 2009 (2009-11-13), pages 190 - 195, XP055029601, ISSN: 0099-2240, DOI: 10.1128/AEM.01772-09
ALBERTYN J ET AL: "GPD1, WHICH ENCODES GLYCEROL 3-PHOSPHATE DEHYDROGENASE, IS ESSENTIAL FOR GROWTH UNDER OSMOTIC STRESS IN SACCHAROMYCES CEREVISIAE, AND ITS EXPRESSION IS REGULATED BY THE HIGH-OSMOLARITY GLYCEROL RESPONSE PATHWAY", MOLECULAR AND CELLULAR BIOLOGY, AMERICAN SOCIETY FOR MICROBIOLOGY, WASHINGTON, US, vol. 14, no. 6, 1 June 1994 (1994-06-01), pages 4135 - 4144, XP000562758, ISSN: 0270-7306
WATANABE T ET AL: "Selection of stress-tolerant yeasts for simultaneous saccharification and fermentation (SSF) of very high gravity (VHG) potato mash to ethanol", BIORESOURCE TECHNOLOGY, ELSEVIER BV, GB, vol. 101, no. 24, 1 December 2010 (2010-12-01), pages 9710 - 9714, XP027246719, ISSN: 0960-8524, [retrieved on 20100724]
VĂCTOR GUADALUPE-MEDINA ET AL: "Evolutionary engineering of a glycerol-3-phosphate dehydrogenase-negative, acetate-reducing Saccharomyces cerevisiae strain enables anaerobic growth at high glucose concentrations", MICROBIAL BIOTECHNOLOGY, vol. 7, no. 1, 4 September 2013 (2013-09-04), pages 44 - 53, XP055096283, ISSN: 1751-7915, DOI: 10.1111/1751-7915.12080
G. HUBMANN ET AL: "Gpd1 and Gpd2 Fine-Tuning for Sustainable Reduction of Glycerol Formation in Saccharomyces cerevisiae", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, vol. 77, no. 17, 1 July 2011 (2011-07-01), pages 5857 - 5867, XP055134657, ISSN: 0099-2240, DOI: 10.1128/AEM.05338-11
V. G. MEDINA ET AL: "Elimination of Glycerol Production in Anaerobic Cultures of a Saccharomyces cerevisiae Strain Engineered To Use Acetic Acid as an Electron Acceptor", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, vol. 76, no. 1, 13 November 2009 (2009-11-13), pages 190 - 195, XP055029601, ISSN: 0099-2240, DOI: 10.1128/AEM.01772-09
ALBERTYN J ET AL: "GPD1, WHICH ENCODES GLYCEROL 3-PHOSPHATE DEHYDROGENASE, IS ESSENTIAL FOR GROWTH UNDER OSMOTIC STRESS IN SACCHAROMYCES CEREVISIAE, AND ITS EXPRESSION IS REGULATED BY THE HIGH-OSMOLARITY GLYCEROL RESPONSE PATHWAY", MOLECULAR AND CELLULAR BIOLOGY, AMERICAN SOCIETY FOR MICROBIOLOGY, WASHINGTON, US, vol. 14, no. 6, 1 June 1994 (1994-06-01), pages 4135 - 4144, XP000562758, ISSN: 0270-7306
WATANABE T ET AL: "Selection of stress-tolerant yeasts for simultaneous saccharification and fermentation (SSF) of very high gravity (VHG) potato mash to ethanol", BIORESOURCE TECHNOLOGY, ELSEVIER BV, GB, vol. 101, no. 24, 1 December 2010 (2010-12-01), pages 9710 - 9714, XP027246719, ISSN: 0960-8524, [retrieved on 20100724]
VĂCTOR GUADALUPE-MEDINA ET AL: "Evolutionary engineering of a glycerol-3-phosphate dehydrogenase-negative, acetate-reducing Saccharomyces cerevisiae strain enables anaerobic growth at high glucose concentrations", MICROBIAL BIOTECHNOLOGY, vol. 7, no. 1, 4 September 2013 (2013-09-04), pages 44 - 53, XP055096283, ISSN: 1751-7915, DOI: 10.1111/1751-7915.12080
Attorney, Agent or Firm:
KLEIBORN, Paul Erik (P.O. Box 4, AA Echt, NL)
Download PDF:
Previous Patent: AQUEOUS GEL PREPARATIONS HAVING DIETHYL ETHER
Next Patent: PROCESS FOR PRODUCING SUSPENSIONS
Next Patent: PROCESS FOR PRODUCING SUSPENSIONS