Title:
GROUP OF ISOTOPE LABELLING REAGENTS FOR CARBOXYL LABELLING AND SYNTHESIS METHOD THEREFOR
Document Type and Number:
WIPO Patent Application WO/2018/176253
Kind Code:
A1
Abstract:
Provided are a group of isotope labelling reagents for carboxyl labelling and a synthesis method therefor, wherein one end of the isotope labelling reagent is a primary amine and the other end is a trimethyl ammonium salt; and the primary amine and the trimethyl ammonium salt are connected by means of a plurality of carbon chains. The synthesis process involves: protecting primary amino groups, with N,N-dimethylethylenediamine and homologues thereof as reaction raw materials, by using di-tert-butyl dicarbonate ((BOC)2O), and then reacting same with a methyl iodide reagent which contains isotope D and 13C under alkaline conditions, and finally removing the protective group under acidic conditions to obtain a target product. By means of a nucleophilic substitution reaction, a group of isotope labelling reagents which can be used for the high-efficiency labelling of carboxyl groups are synthesised; the price is low, the operation is simple, and after labelling with the isotope reagent, the efficiency and accuracy of analysis can be significantly improved.
Inventors:
WU MINGHUO (CN)
LU MIN (CN)
ZHOU HAO (CN)
HU YUFENG (CN)
LU MIN (CN)
ZHOU HAO (CN)
HU YUFENG (CN)
Application Number:
PCT/CN2017/078547
Publication Date:
October 04, 2018
Filing Date:
March 29, 2017
Export Citation:
Assignee:
UNIV DALIAN TECH (CN)
International Classes:
C07C211/63; C07B59/00; C07C209/62
Domestic Patent References:
| WO2007109292A2 | 2007-09-27 |
Foreign References:
| CN107011185A | 2017-08-04 |
Other References:
TORDE, RICHARD G: "Multiplexed Analysis of Cage and Cage Free Chicken Egg Fatty Acids Using Stable Isotope Labeling and Mass Spectrometry", MOLECULES, vol. 18, no. 12, 5 December 2013 (2013-12-05), pages 14977 - 14988, XP055539749, ISSN: 1420-3049
LAMOS, SHANE M: "Relative Quantification of Carboxylic Acid Metabolites by Liquid Chromatography-Mass Spectrometry Using Isotopic Variants of Cholamine", ANAL. CHEM., vol. 79, no. 14, July 2007 (2007-07-01), pages 5143 - 5149, XP055539751, ISSN: 1520-6882
LAMOS, SHANE M: "Relative Quantification of Carboxylic Acid Metabolites by Liquid Chromatography-Mass Spectrometry Using Isotopic Variants of Cholamine", ANAL. CHEM., vol. 79, no. 14, July 2007 (2007-07-01), pages 5143 - 5149, XP055539751, ISSN: 1520-6882
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