Title:
GROWTH OF FILTERABLE ORGANISMS THAT REQUIRE NON-DIFFUSABLE MEDIA COMPONENTS
Document Type and Number:
WIPO Patent Application WO2002097052
Kind Code:
A3
Abstract:
Provided are membrane filters and methods of trapping, and culturing microorganisms. The membrane filters trap or filter from a sample a specific microorganism of interest requiring a set of growth components. The membrane comprises a first subset of the set of growth components such that the microorganism can grow and divide with the membrane contacting a medium comprising a second subset of the set of growth components defined as all components of the set of growth components not included in the first subset. Methods for detecting a microorganism comprise: (i) providing a membrane filter, comprising a first subset of the set of growth components; (ii) filtering a sample through the filter; and (iii) placing the filter in contact with a medium comprising a second subset of the set of components for growth. For members of genus Legionella a filter impregnated with activated charcoal may be employed for detection and culturing.
Inventors:
HALL JANE H
BORCHARDT KENNETH
HALL ROBERT D
BORCHARDT KENNETH
HALL ROBERT D
Application Number:
PCT/US2002/017025
Publication Date:
March 11, 2004
Filing Date:
May 28, 2002
Export Citation:
Assignee:
BIOMED DIAGNOSTICS (US)
International Classes:
C12N11/14; C12Q1/04; C12Q1/24; (IPC1-7): C12Q1/02
Domestic Patent References:
| WO2000023792A1 | 2000-04-27 |
Foreign References:
| US5415994A | 1995-05-16 | |||
| US5208161A | 1993-05-04 |
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